Abstract：
Disintegrins, a family of snake venom protein, which are capable of modulating the activity of integrins that play a fundamental role in the regulation of many physiological and pathological processes. The main purpose of this study is to obtain the recombinant disintegrin (r-DI) and evaluate its biological activity. In this study, we explored a high-level expression prokaryotic system and purification strategy for r-DI. Then, r-DI was treated to assay effects on cell growth, migration, and invasion. The affinity for the interactions of r-DI with integrin was determined using Surface plasmon resonance (SPR) analyses. The r-DI can be expressed in Escherichia coli and purified by one-step chromatography. The r-DI can inhibit B16F10 cells proliferation, migration, and invasion. Also, we found that r-DI could interact with the integrin αIIbβ3 (GPIIb/IIIa). The r-DI can be expressed, purified, characterized through functional assays, and can also maintain strong biological activities. Thus, this study showed potential therapeutic effects of r-DI for further functional and structural studies.
摘要：
Disintegrins,一个蛇毒蛋白家族,它们能够调节在许多生理和病理过程的调节中起基本作用的整合素的活性。本研究的主要目的是获得重组解整合素（r-DI）并评估其生物学活性。在这项研究中,我们探索了r-DI的高水平表达原核系统和纯化策略。然后，处理r-DI以测定对细胞生长的影响，迁移,和入侵。使用表面等离子体共振（SPR）分析确定r-DI与整联蛋白相互作用的亲和力。r-DI可以在大肠杆菌中表达并通过一步色谱法纯化。r-DI可抑制B16F10细胞增殖,迁移,和入侵。此外，我们发现r-DI可以与整合素αIIbβ3（GPIIb/IIIa）相互作用。r-DI可以表示为，纯化,通过功能测定表征，还可以保持强大的生物活性。因此，这项研究显示了r-DI在进一步的功能和结构研究中的潜在治疗作用.
