PDF(Pubmed)
Abstract：
Currently, a clear interest has been given to berries due to their richness in active metabolites, including anthocyanins and non-coloured phenolics. Therefore, the main aim of the present work is to investigate the phenolic profile, antioxidant abilities, and antiproliferative effects on normal human dermal fibroblasts (NHDF) and human colon carcinoma cell line (Caco-2) cells of phenolic-rich extracts from three red fruits highly appreciated by consumers: two species of blackberries (Rubus fruticosus and Rubus ulmifolius) and one species of mulberry (Morus nigra). A total of 19 different phenolics were identified and quantified by HPLC-DAD-ESI/MSn and HPLC-DAD, respectively. Focusing on the biological potential of the phenolic-rich extracts, all of them revealed notable scavenging abilities. Concerning the antiproliferative properties, R. fruticosus presented a cytotoxic selectivity for Caco-2 cells compared to NHDF cells. To deeper explore the biological potential, combinations with positive controls (ascorbic acid and 5-fluorouracil) were also conducted. Finally, the obtained data are another piece of evidence that the combination of phenolic-rich extracts from natural plants with positive controls may reduce clinical therapy costs and the possible toxicity of chemical drugs.
摘要：
目前,一个明确的兴趣已经给了浆果由于其丰富的活性代谢产物，包括花青素和无色酚类。因此,本工作的主要目的是研究酚醛的概况，抗氧化能力，来自消费者高度赞赏的三种红色水果的富含酚类提取物对正常人真皮成纤维细胞（NHDF）和人结肠癌细胞系（Caco-2）细胞的抗增殖作用：两种黑莓（Rubusfruticosus和Rubusulmifolius）和一种桑树（Morusnigra）。通过HPLC-DAD-ESI/MSn和HPLC-DAD鉴定并定量了19种不同的酚类物质，分别。专注于富含酚类提取物的生物潜力，他们都表现出明显的清除能力。关于抗增殖特性，与NHDF细胞相比，fruticosusR.表现出对Caco-2细胞的细胞毒性选择性。为了更深入地探索生物潜力，还进行了与阳性对照(抗坏血酸和5-氟尿嘧啶)的组合。最后,获得的数据是另一个证据,表明天然植物富含酚类的提取物与阳性对照的组合可以降低临床治疗成本和化学药物可能的毒性.
