PDF(Pubmed)
Abstract：
Human milk oligosaccharides (HMOs) are complexes that play a crucial role in shaping the early-life gut microbiota. This study intends to explore whether HMO patterns are associated with the gut microbiota of infants. We included 96 Chinese breastfeeding mother-infant dyads. Breast milk and infant faecal samples were collected and tested. With milk 2\'-fucosyllactose, difucosyllactose, and lacto-N-fucopentaose-I as biomarkers, we divided the mothers into secretor and non-secretor groups. HMO patterns were extracted using principal component analysis. The majority (70.7%) of mothers were categorised as secretor and five different HMO patterns were identified. After adjustment, the infants of secretor mothers exhibited a lower relative abundance of Bifidobacterium bifidum (β = -0.245, 95%CI: -0.465~-0.025). An HMO pattern characterised by high levels of 3-fucosyllactose, lacto-N-fucopentaose-III, and lacto-N-neodifucohexaose-II was positively associated with the relative abundance of Bifidobacterium breve (p = 0.014), while the pattern characterised by lacto-N-neotetraose, 6\'-sialyllactose, and sialyllacto-N-tetraose-b was negatively associated with Bifidobacterium breve (p = 0.027). The pattern characterised by high levels of monofucosyl-lacto-N-hexaose-III and monofucosyl-lacto-N-neohexaose was positively associated with Bifidobacterium dentium (p = 0.025) and Bifidobacterium bifidum (p < 0.001), respectively. This study suggests that HMO patterns from mature breast milk were associated with certain gut microbiota of breastfed infants.
摘要：
人乳寡糖（HMO）是在塑造早期肠道微生物群中发挥关键作用的复合物。这项研究旨在探索HMO模式是否与婴儿的肠道菌群相关。我们纳入了96个中国母乳喂养的母婴二叉。收集母乳和婴儿粪便样品并进行测试。用牛奶2'-岩藻糖基乳糖，双岩藻糖基乳糖,和乳-N-岩藻五糖-I作为生物标志物，我们把母亲分成了分泌者和非分泌者组。使用主成分分析提取HMO模式。大多数（70.7％）的母亲被归类为分泌者，并确定了五种不同的HMO模式。调整后,分泌母亲的婴儿显示两歧双歧杆菌的相对丰度较低（β=-0.245，95CI：-0.465〜-0.025）。以高水平的3-岩藻糖基乳糖为特征的HMO模式，乳酸-N-岩藻五糖-III,乳-N-新二岩藻六糖-II与短双歧杆菌的相对丰度呈正相关（p=0.014），虽然这种模式的特征是lacto-N-neotetraose，6\'-唾液酸乳糖，唾液酸-N-四糖-b与短双歧杆菌呈负相关(p=0.027)。以高水平的单岩藻糖基-乳酸-N-六糖-III和单岩藻糖基-乳酸-N-新六糖为特征的模式与双歧杆菌（p=0.025）和双歧杆菌（p<0.001）呈正相关，分别。这项研究表明，成熟母乳中的HMO模式与母乳喂养婴儿的某些肠道微生物群有关。
