PDF(Pubmed)
Abstract：
BACKGROUND: The current manuscript\'s aim was to determine the human papillomavirus (HPV) genotype-specific prevalence and distribution among individuals, males, and females, of different ages in the region of Apulia, Italy, highlighting the possible variables involved in the carcinogenicity mechanism. In addition, we proposed two hypothetical models of HPV\'s molecular dynamics, intending to clarify the impact of prevention and therapeutic strategies, explicitly modeled by recent survey data.
METHODS: We presented clinical data from 9647 participants tested for either high-risk (HR) or low-risk (LR) HPV at the affiliated Bari Policlinic University Hospital of Bari from 2011 to 2022. HPV DNA detection was performed using nested-polymerase chain reaction (PCR) and multiplex real-time PCR assay. Statistical analysis showed significant associations for all genders and ages and both HR- and LR-HPV types. A major number of significant pairwise associations were detected for the higher-risk types and females and lower-risk types and males.
RESULTS: The overall prevalence of HPV was 50.5% (n-4.869) vs. 49.5% (n-4.778) of the study population, of which 74.4% (n-3621) were found to be HPV high-risk (HR-HPV) genotypes and 57.7% (n-2.807) low-risk HPV (LR-HPV) genotypes, of which males were 58% and females 49%; the three most prevalent HR-HPV genotypes were HPV 53 (n707-15%), 16 (n704-14%), and 31 (n589-12%), and for LR-HPV, they were 42 (19%), 6 (16%), and 54 (13%); 56% of patients screened for HPV were ≤ 30 years old, 53% were between 31 and 40 years old, 46% were 41-50 and 51-60 years old, and finally, 44% of subjects were >60 years old.
CONCLUSIONS: Our study provided comprehensive epidemiological data on HPV prevalence and genotype distribution among 9647 participants, which could serve as a significant reference for clinical practice, and it implied the necessity for more effective screening methods for HPV carcinogenesis covering the use of more specific molecular investigations. Although this is a predominantly descriptive and epidemiological study, the data obtained offer not only a fairly unique trend compared to other studies of different realities and latitudes but also lead us to focus on the HPV infection within two groups of young people and adults and hypothesize the possible involvement of dysbiosis, stem cells, and the retrotransposition mechanism.
METHODS: We presented clinical data from 9647 participants tested for either high-risk (HR) or low-risk (LR) HPV at the affiliated Bari Policlinic University Hospital of Bari from 2011 to 2022. HPV DNA detection was performed using nested-polymerase chain reaction (PCR) and multiplex real-time PCR assay. Statistical analysis showed significant associations for all genders and ages and both HR- and LR-HPV types. A major number of significant pairwise associations were detected for the higher-risk types and females and lower-risk types and males.
RESULTS: The overall prevalence of HPV was 50.5% (n-4.869) vs. 49.5% (n-4.778) of the study population, of which 74.4% (n-3621) were found to be HPV high-risk (HR-HPV) genotypes and 57.7% (n-2.807) low-risk HPV (LR-HPV) genotypes, of which males were 58% and females 49%; the three most prevalent HR-HPV genotypes were HPV 53 (n707-15%), 16 (n704-14%), and 31 (n589-12%), and for LR-HPV, they were 42 (19%), 6 (16%), and 54 (13%); 56% of patients screened for HPV were ≤ 30 years old, 53% were between 31 and 40 years old, 46% were 41-50 and 51-60 years old, and finally, 44% of subjects were >60 years old.
CONCLUSIONS: Our study provided comprehensive epidemiological data on HPV prevalence and genotype distribution among 9647 participants, which could serve as a significant reference for clinical practice, and it implied the necessity for more effective screening methods for HPV carcinogenesis covering the use of more specific molecular investigations. Although this is a predominantly descriptive and epidemiological study, the data obtained offer not only a fairly unique trend compared to other studies of different realities and latitudes but also lead us to focus on the HPV infection within two groups of young people and adults and hypothesize the possible involvement of dysbiosis, stem cells, and the retrotransposition mechanism.
摘要：
背景：当前手稿的目的是确定人乳头瘤病毒（HPV）基因型特异性患病率和个体之间的分布，男性,和女性，普利亚地区不同年龄的人，意大利,强调参与致癌机制的可能变量。此外,我们提出了两种假设的HPV分子动力学模型，旨在阐明预防和治疗策略的影响，由最近的调查数据明确建模。
方法：我们提供了2011年至2022年在巴里附属巴里Policlinic大学附属医院检测高风险（HR）或低风险（LR）HPV的9647名参与者的临床数据。使用巢式聚合酶链反应（PCR）和多重实时PCR测定法进行HPVDNA检测。统计分析显示所有性别和年龄以及HR和LR-HPV类型的显着关联。在高风险类型和女性以及低风险类型和男性中，发现了大量显着的成对关联。
结果：HPV的总体患病率为50.5%（n-4.869）与49.5%(n=4.778)的研究人群，其中74.4%（n-3621）为HPV高危型（HR-HPV）基因型，57.7%（n-2.807）为低危型HPV（LR-HPV）基因型，其中男性占58％，女性占49％;三种最普遍的HR-HPV基因型是HPV53（n707-15％），16（n704-14%），和31（n589-12%），对于LR-HPV，他们是42（19％），6（16%），54（13％）;56％的HPV筛查患者≤30岁，53%的人年龄在31至40岁之间，46％为41-50岁和51-60岁，最后，44%的受试者年龄>60岁。
结论：我们的研究提供了9647名参与者的HPV患病率和基因型分布的全面流行病学数据，这可以作为临床实践的重要参考，这意味着需要更有效的HPV癌变筛查方法，包括使用更具体的分子研究。尽管这是一项主要的描述性和流行病学研究，与其他不同现实和纬度的研究相比，获得的数据不仅提供了相当独特的趋势，而且还使我们专注于两组年轻人和成年人中的HPV感染，并假设可能涉及菌群失调，干细胞,和逆转换位机制。
方法：我们提供了2011年至2022年在巴里附属巴里Policlinic大学附属医院检测高风险（HR）或低风险（LR）HPV的9647名参与者的临床数据。使用巢式聚合酶链反应（PCR）和多重实时PCR测定法进行HPVDNA检测。统计分析显示所有性别和年龄以及HR和LR-HPV类型的显着关联。在高风险类型和女性以及低风险类型和男性中，发现了大量显着的成对关联。
结果：HPV的总体患病率为50.5%（n-4.869）与49.5%(n=4.778)的研究人群，其中74.4%（n-3621）为HPV高危型（HR-HPV）基因型，57.7%（n-2.807）为低危型HPV（LR-HPV）基因型，其中男性占58％，女性占49％;三种最普遍的HR-HPV基因型是HPV53（n707-15％），16（n704-14%），和31（n589-12%），对于LR-HPV，他们是42（19％），6（16%），54（13％）;56％的HPV筛查患者≤30岁，53%的人年龄在31至40岁之间，46％为41-50岁和51-60岁，最后，44%的受试者年龄>60岁。
结论：我们的研究提供了9647名参与者的HPV患病率和基因型分布的全面流行病学数据，这可以作为临床实践的重要参考，这意味着需要更有效的HPV癌变筛查方法，包括使用更具体的分子研究。尽管这是一项主要的描述性和流行病学研究，与其他不同现实和纬度的研究相比，获得的数据不仅提供了相当独特的趋势，而且还使我们专注于两组年轻人和成年人中的HPV感染，并假设可能涉及菌群失调，干细胞,和逆转换位机制。
