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Abstract：
We aimed to determine SARS-CoV-2 antibody seropositivity among pregnant women and the transplacental transfer efficiency of SARS-CoV-2-specific antibodies relative to malaria antibodies among SARS-CoV-2 seropositive mother-cord pairs. This cross-sectional study was conducted in Accra, Ghana, from March to May 2022. Antigen- specific IgG antibodies against SARS-CoV-2 (nucleoprotein and spike-receptor binding domain) and malarial antigens (circumsporozoite protein and merozoite surface protein 3) in maternal and cord plasma were measured by ELISA. Plasma from both vaccinated and unvaccinated pregnant women were tested for neutralizing antibodies using commercial kit. Of the unvaccinated pregnant women tested, 58.12% at antenatal clinics and 55.56% at the delivery wards were seropositive for both SARS-CoV-2 nucleoprotein and RBD antibodies. Anti-SARS-CoV-2 antibodies in cord samples correlated with maternal antibody levels (N antigen rs = 0.7155, p < 0.001; RBD rs = 0.8693, p < 0.001). Transplacental transfer of SARS-CoV-2 nucleoprotein antibodies was comparable to circumsporozoite protein antibodies (p = 0.9999) but both were higher than transfer rates of merozoite surface protein 3 antibodies (p < 0.001). SARS-CoV-2 IgG seropositivity among pregnant women in Accra is high with a boost of SARS-CoV-2 RBD-specific IgG in vaccinated women. Transplacental transfer of anti-SARS-CoV-2 and malarial antibodies was efficient, supporting vaccination of mothers as a strategy to protect infants against SARS-CoV-2.
摘要：
我们旨在确定孕妇的SARS-CoV-2抗体血清阳性，以及SARS-CoV-2特异性抗体相对于SARS-CoV-2血清阳性母带对中疟疾抗体的胎盘转移效率。这项横断面研究是在阿克拉进行的，加纳,2022年3月至5月通过ELISA测量母体和脐带血浆中针对SARS-CoV-2（核蛋白和刺突受体结合域）和疟疾抗原（环子孢子蛋白和裂殖子孢子表面蛋白3）的抗原特异性IgG抗体。使用商业试剂盒测试来自接种疫苗和未接种疫苗的孕妇的血浆中和抗体。在接受测试的未接种疫苗的孕妇中，产前诊所的58.12％和分娩病房的55.56％的SARS-CoV-2核蛋白和RBD抗体均为血清阳性。脐带样本中的抗SARS-CoV-2抗体与母体抗体水平相关(N抗原rs=0.7155,p<0.001;RBDrs=0.8693,p<0.001)。SARS-CoV-2核蛋白抗体的胎盘转移与环子孢子蛋白抗体相当（p=0.9999），但均高于裂殖子孢子表面蛋白3抗体的转移速率（p<0.001）。阿克拉孕妇的SARS-CoV-2IgG血清阳性率很高，接种疫苗的妇女中SARS-CoV-2RBD特异性IgG的增加。抗SARS-CoV-2和疟疾抗体的胎盘转移是有效的，支持母亲接种疫苗，作为保护婴儿免受SARS-CoV-2感染的策略。
