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Abstract：
The first electrochemical sensor application in the literature is described for the sensitive and selective determination of the selective Janus kinase (JAK)-1 inhibitor abrocitinib (ABR). ABR is approved by the U.S. Food and Drug Administration (FDA) for the treatment of atopic dermatitis. The molecularly imprinted polymer (MIP)-based sensor was designed to incorporate zinc nanoflower (ZnNFs)-graphene oxide (GO) conjugate (ZnNFs@GO), synthesized from the root methanolic extract (RME) of the species Alkanna cappadocica Boiss. et Bal. to improve the porosity and effective surface area of the glassy carbon electrode (GCE). Furthermore, the MIP structure was prepared using ABR as a template molecule, 4-aminobenzoic acid (4-ABA) as a functional monomer, and other additional components. Scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) were used to characterize the surface and structure of the synthesized nanomaterial and MIP-based surface. Among the electrochemical methods, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were preferred for detailed electrochemical characterization, and differential pulse voltammetry (DPV) was preferred for all other electrochemical measurements using 5.0 mM [Fe(CN)6]3-/4- solution as the redox probe. The MIP-based sensor, which was the result of a detailed optimization phase, gave a linear response in the 1.0 × 10-13 - 1.0 × 10-12 M range in standard solution and serum sample. The obtained limit of detection (LOD) and limit of quantification (LOQ) values and recovery studies demonstrated the sensitivity, accuracy, and applicability of the sensor. Selectivity, the most important feature of the MIP-based sensor, was verified by imprinting factor calculations using ibrutinib, ruxolitinib, tofacitinib, zonisamide, and acetazolamide.
摘要：
文献中的第一个电化学传感器应用被描述用于选择性Janus激酶(JAK)-1抑制剂abrocitinib(ABR)的灵敏和选择性测定。ABR已获得美国食品和药物管理局（FDA）的批准，用于治疗特应性皮炎。设计了基于分子印迹聚合物（MIP）的传感器，以结合锌纳米花（ZnNFs）-氧化石墨烯（GO）缀合物（ZnNFs@GO），由AlkannacappadocicaBoiss物种的根甲醇提取物（RME）合成。etBal.提高玻碳电极(GCE)的孔隙率和有效表面积。此外，使用ABR作为模板分子制备MIP结构，4-氨基苯甲酸（4-ABA）作为功能单体，和其他附加组件。扫描电子显微镜（SEM），X射线衍射(XRD)用傅里叶变换红外光谱(FTIR)对合成的纳米材料和MIP基表面进行了表征。在电化学方法中，循环伏安法（CV）和电化学阻抗谱（EIS）是详细的电化学表征的首选，对于使用5.0mM[Fe(CN)6]3-/4-溶液作为氧化还原探针的所有其他电化学测量,差分脉冲伏安法(DPV)是优选的。基于MIP的传感器，这是详细优化阶段的结果，在标准溶液和血清样品中，在1.0×10-13-1.0×10-12M范围内给出线性响应。获得的检测限（LOD）和定量限（LOQ）值和回收率研究表明了灵敏度，准确度,和传感器的适用性。选择性,基于MIP的传感器最重要的功能，通过使用ibrutinib的印记因子计算验证，鲁索替尼,托法替尼,唑尼沙胺，还有乙酰唑胺.
