二甲基2-[2-(1-苯基-4,5-二氢-1H-咪唑-2-基)肼基]丁二醇酯(DIHB)和8-(3-氯苯基)-2,6,7,8-四氢咪唑并[2,1-c][1,2,4]三嗪-3,4-二酮(HDIT)是有希望的抗癌剂候选物,本文介绍了其中的第一个分析程序。市售未修饰的玻碳电极(GCE)用作传感器,用于单独和同时差分脉冲伏安法(DPV)测定这些可能的抗癌药物。有关电化学行为的发现表明DIHB和HDIT在GCE上显示,作为一个传感器,氧化峰在1.18和0.98V,分别(与Ag/AgCl,3.0molL-1KCl)在pH=4.5的0.125molL-1乙酸盐缓冲液中,用于定量。仔细研究了各种实验参数,在伏安测量中实现高灵敏度。最后,在优化条件下(t为60s,ΔEA为75mV,ν为225mVs-1,tm为2ms),GCE提出的DPV程序显示出广泛的线性传感范围(1-200nmolL-1-DIHB和5-200nmolL-1-HDIT),DIHB的检出限为0.18nmolL-1,HDIT的检出限为1.1nmolL-1。此外,开发的程序具有良好的选择性,DIHB和HDIT信号的可重复性和传感器的再现性。通过分析尿液参考材料而无需任何事先处理,证明了该方法的实际应用。结果表明,这种环境友好的方法,带有免修改的传感器,适用于敏感的,DIHB和HDIT的选择性和快速定量。
Dimethyl 2-[2-(1-phenyl-4,5-dihydro-1H-imidazol-2-yl)hydrazinylidene]butanedioate (DIHB) and 8-(3-chlorophenyl)-2,6,7,8-tetrahydroimidazo[2,1-c][1,2,4]triazine-3,4-dione (HDIT) are promising candidates for anticancer agents, the first analytical procedures of which are presented in this paper. The commercially available unmodified glassy carbon electrode (GCE) was used as a sensor for the individual and simultaneous differential pulse voltammetric (DPV) determination of these possible anticancer drugs. The findings concerning the electrochemical behaviour indicated that DIHB and HDIT display at GCE, as a sensor, the oxidation peaks at 1.18 and 0.98 V, respectively (vs. Ag/AgCl, 3.0 mol L-1 KCl) in the 0.125 mol L-1 acetate buffer of pH = 4.5, which were employed for their quantification. Various experimental parameters were carefully investigated, to achieve high sensitivity in voltammetric measurements. Finally, under the optimised conditions (t of 60 s, ΔEA of 75 mV, ν of 225 mV s-1, and tm of 2 ms), the proposed DPV procedure with the GCE demonstrated broad linear sensing ranges (1-200 nmol L-1-DIHB and 5-200 nmol L-1-HDIT), boasting the detection limits of 0.18 nmol L-1 for DIHB and 1.1 nmol L-1 for HDIT. Moreover, the developed procedure was distinguished by good selectivity, repeatability of DIHB and HDIT signals and sensor reproducibility. The practical application of this method was demonstrated by analysing the urine reference material without any prior treatment. The results showed that this environmentally friendly approach, with a modification-free sensor, is suitable for the sensitive, selective and rapid quantification of DIHB and HDIT.