RESULTS: We employed RNA-Seq to sequence three distinct tissues (leaf, root, and stem) of P. kingianum. The analysis revealed a total of 31,558 differentially expressed genes (DEGs), with 43 species of transcription factors annotated among them. Analyses via gene ontology and the Kyoto Encyclopedia of Genes and Genomes demonstrated that these DEGs were predominantly enriched in metabolic pathways and secondary metabolite biosynthesis. The proposed temporal expression analysis categorized the DEGs into nine clusters, suggesting the same expression trends that may be coordinated in multiple biological processes across the three tissues. Additionally, we conducted screening and expression pattern analysis of the ARF gene family, identifying 12 significantly expressed PkARF genes in P. kingianum roots. This discovery lays the groundwork for investigations into the role of PkARF genes in root growth, development, and secondary metabolism regulation.
CONCLUSIONS: The obtained data and insights serve as a focal point for further research studies, centred on genetic manipulation of growth and secondary metabolism in P. kingianum. Furthermore, these findings contribute to the understanding of functional genomics in P. kingianum, offering valuable genetic resources.
结果:我们使用RNA-Seq对三种不同的组织(叶,根,和茎)。分析显示总共31,558个差异表达基因(DEGs),其中注释了43种转录因子。通过基因本体论和京都基因和基因组百科全书的分析表明,这些DEGs主要富集在代谢途径和次生代谢产物的生物合成中。拟议的时间表达分析将DEG分为9个簇,表明相同的表达趋势,可能在三个组织的多个生物过程中协调。此外,我们进行了ARF基因家族的筛选和表达模式分析,鉴定了12个显著表达的PkARF基因。这一发现为研究PkARF基因在根系生长中的作用奠定了基础。发展,和次级代谢调节。
结论:获得的数据和见解是进一步研究的重点,集中在对P.kingianum的生长和次生代谢的遗传操作。此外,这些发现有助于理解金鸡假单胞菌的功能基因组学,提供宝贵的遗传资源。