背景:根据中国古籍,黄精的果实和根茎都具有药用和食用价值。到目前为止,目前还没有关于沙棘果实和不同年生根茎的代谢产物谱和调控网络的报道。
结果:在这项研究中,我们进行了代谢组和转录组的综合分析,以揭示果实和不同年生根茎的动态积累和调节网络。酚酸的相对含量,木脂素和香豆素,黄酮类化合物和生物碱随着生长年限的增加而增加,而类固醇和脂质则随之减少。此外,核苷酸和衍生物的相对含量,黄酮类化合物,有机酸,果实中的类固醇和脂质高于根茎。可能与多糖生物合成有关的基因,黄酮类化合物,通过转录组分析进一步分析了三萜皂苷和生物碱的生物合成,包括sacA,GMPP,PMM,CCoAOMT,CHI,ANR,CHS,DXS,GGPS,ZEP,CYP72A219等,其表达量与代谢物的相对含量呈正相关。此外,构建了糖和芳香族氨基酸代谢产物的相关网络,以进一步说明多糖的生物合成,黄酮类化合物和生物碱,并筛选了一些转录因子(TFs),如C2C2,MYB,bZIP,GRAS和NAC。
结论:这项研究可以加深我们对P.cyrtonema中主要化合物的积累模式和分子机制的理解,为球藻的规范化生产提供参考。
BACKGROUND: According to Chinese ancient books, both fruits and rhizomes of
Polygonatum cyrtonema Hua have medicinal and edible values. Up to now, there is no report about the metabolite profiles and regulatory network in fruits and different year-old rhizomes of P. cyrtonema.
RESULTS: In this study, we performed integrative analyses of metabolome and transcriptome to reveal the dynamic accumulation and regulatory network of fruits and different year-old rhizomes in P. cyrtonema. The relative content of phenolic acids, lignans and coumarins, flavonoids and alkaloids increased with growth years, while steroids and lipids decreased with it. In addition, the relative content of nucleotides and derivatives, flavonoids, organic acids, steroids and lipids in fruits were higher than rhizomes. Genes that might relate to the biosynthesis of polysaccharides, flavonoids, triterpene saponins and alkaloids biosynthesis were further analyzed by transcriptome analysis, including sacA, GMPP, PMM, CCoAOMT, CHI, ANR, CHS, DXS, GGPS, ZEP, CYP72A219 and so on, for their expressions were positively correlated with the relative content of the metabolites. Additionally, the correlation network in sugar and aromatic amino acids metabolites were constructed to further illustrate the biosynthesis of polysaccharides, flavonoids and alkaloids in P. cyrtonema, and some transcription factors (TFs) were screened, such as C2C2, MYB, bZIP, GRAS and NAC.
CONCLUSIONS: This study can deepen our understanding of the accumulation patterns and molecular mechanism of the main compounds in P. cyrtonema, and provide reference for the standardize production of P. cyrtonema.