Abstract：
BACKGROUND: Our aim was to establish which of the current and novel storage fluids caused the least harm to the endothelium of the saphenous vein.
METHODS: Unmanipulated samples of saphenous vein were obtained. This vein was exposed to four fluids, DuraGraft®, Plasma-Lyte, autologous blood and sodium chloride, and a control medium. The expression of endothelial selectin (E-selectin) and endothelial nitric oxide synthase (eNOS) was measured using confocal microscopy.
RESULTS: We identified and quantified platelet endothelial cell adhesion molecules, vascular cell adhesion molecules and endothelial nitric oxide synthase 3 on the endothelium. The results were widely variable. The protein expression of endothelial selectin (p = .17) and endothelial nitric oxide synthase 3 (p = .73) showed no statistically significant differences in levels of preservation when the different solutions were compared.
CONCLUSIONS: In this study, we could not determine that any of the solutions were superior at preserving saphenous vein endothelium.
METHODS: Unmanipulated samples of saphenous vein were obtained. This vein was exposed to four fluids, DuraGraft®, Plasma-Lyte, autologous blood and sodium chloride, and a control medium. The expression of endothelial selectin (E-selectin) and endothelial nitric oxide synthase (eNOS) was measured using confocal microscopy.
RESULTS: We identified and quantified platelet endothelial cell adhesion molecules, vascular cell adhesion molecules and endothelial nitric oxide synthase 3 on the endothelium. The results were widely variable. The protein expression of endothelial selectin (p = .17) and endothelial nitric oxide synthase 3 (p = .73) showed no statistically significant differences in levels of preservation when the different solutions were compared.
CONCLUSIONS: In this study, we could not determine that any of the solutions were superior at preserving saphenous vein endothelium.
摘要：
背景：我们的目的是确定当前和新型存储液中哪种对隐静脉的内皮造成的伤害最小。
方法:获得未操作的隐静脉样品。这条静脉暴露在四种液体中,DuraGraft®,血浆-Lyte,自体血和氯化钠，和控制媒介。使用共聚焦显微镜测量内皮选择素（E-selectin）和内皮一氧化氮合酶（eNOS）的表达。
结果：我们鉴定并定量了血小板内皮细胞粘附分子，血管内皮细胞粘附分子和内皮型一氧化氮合酶3。结果变化很大。当比较不同的溶液时，内皮选择素（p=.17）和内皮一氧化氮合酶3（p=.73）的蛋白表达在保存水平上没有统计学意义。
结论：在这项研究中，我们无法确定任何一种解决方案在保留隐静脉内皮方面都是优越的。
方法:获得未操作的隐静脉样品。这条静脉暴露在四种液体中,DuraGraft®,血浆-Lyte,自体血和氯化钠，和控制媒介。使用共聚焦显微镜测量内皮选择素（E-selectin）和内皮一氧化氮合酶（eNOS）的表达。
结果：我们鉴定并定量了血小板内皮细胞粘附分子，血管内皮细胞粘附分子和内皮型一氧化氮合酶3。结果变化很大。当比较不同的溶液时，内皮选择素（p=.17）和内皮一氧化氮合酶3（p=.73）的蛋白表达在保存水平上没有统计学意义。
结论：在这项研究中，我们无法确定任何一种解决方案在保留隐静脉内皮方面都是优越的。
