{Reference Type}: Journal Article
{Title}: Comparison of storage solutions on saphenous vein endothelial function using immunofluorescence. A pilot study.
{Author}: Danby A;Briffa N;
{Journal}: Perfusion
{Volume}: 0
{Issue}: 0
{Year}: 2024 May 9
{Factor}: 1.581
{DOI}: 10.1177/02676591241253460
{Abstract}: <B>BACKGROUND: </B>Our aim was to establish which of the current and novel storage fluids caused the least harm to the endothelium of the saphenous vein.<BR><B>METHODS: </B>Unmanipulated samples of saphenous vein were obtained. This vein was exposed to four fluids, DuraGraft®, Plasma-Lyte, autologous blood and sodium chloride, and a control medium. The expression of endothelial selectin (E-selectin) and endothelial nitric oxide synthase (eNOS) was measured using confocal microscopy.<BR><B>RESULTS: </B>We identified and quantified platelet endothelial cell adhesion molecules, vascular cell adhesion molecules and endothelial nitric oxide synthase 3 on the endothelium. The results were widely variable. The protein expression of endothelial selectin (p = .17) and endothelial nitric oxide synthase 3 (p = .73) showed no statistically significant differences in levels of preservation when the different solutions were compared.<BR><B>CONCLUSIONS: </B>In this study, we could not determine that any of the solutions were superior at preserving saphenous vein endothelium.