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Abstract：
Sunflower is one of the four major oil crops in the world. \'Zaoaidatou\' (ZADT), the main variety of oil sunflower in the northwest of China, has a short growth cycle, high yield, and high resistance to abiotic stress. However, the ability to tolerate adervesity is limited. Therefore, in this study, we used the retention line of backbone parent ZADT as material to establish its tissue culture and genetic transformation system for new variety cultivating to enhance resistance and yields by molecular breeding. The combination of 0.05 mg/L IAA and 2 mg/L KT in MS was more suitable for direct induction of adventitious buds with cotyledon nodes and the addition of 0.9 mg/L IBA to MS was for adventitious rooting. On this basis, an efficient Agrobacterium tumefaciens-mediated genetic transformation system for ZADT was developed by the screening of kanamycin and optimization of transformation conditions. The rate of positive seedlings reached 8.0%, as determined by polymerase chain reaction (PCR), under the condition of 45 mg/L kanamycin, bacterial density of OD600 0.8, infection time of 30 min, and co-cultivation of three days. These efficient regeneration and genetic transformation platforms are very useful for accelerating the molecular breeding process on sunflower.
摘要：
向日葵是世界四大油料作物之一。\'Zaaidatou\'（ZADT），中国西北地区油葵的主要品种，增长周期短，高产,和对非生物胁迫的高抗性。然而,容忍攻击的能力是有限的。因此,在这项研究中，我们以骨干亲本ZADT的保留系为材料，建立了其组织培养和遗传转化体系，用于新品种的培育，以通过分子育种提高抗性和产量。MS中0.05mg/LIAA和2mg/LKT的组合更适合直接诱导具有子叶节的不定芽，MS中添加0.9mg/LIBA用于不定生根。在此基础上,通过卡那霉素的筛选和转化条件的优化，建立了一种高效的根癌农杆菌介导的ZADT遗传转化体系。幼苗阳性率达8.0%,通过聚合酶链反应（PCR）确定，在45mg/L卡那霉素条件下,细菌密度为OD6000.8，感染时间为30分钟，共培养三天。这些高效的再生和遗传转化平台对于加速向日葵的分子育种过程非常有用。
