Abstract：
The affect of temperature on tissue protein synthesis rates has been reported in temperate and tropical, but not Antarctic fishes. Previous studies have generally demonstrated low growth rates in Antarctic fish species in comparison to temperate relatives and elevated levels of protein turnover. This study investigates how low temperatures effect tissue protein synthesis and hence tissue growth in a polar fish species. Groups of Antarctic, Harpagifer antarcticus and temperate, Lipophrys pholis, were acclimated to a range of overlapping water temperatures and protein synthesis was measure in white muscle (WM), liver and gastrointestinal tract (GIT). WM protein synthesis rates increased linearly with temperature in both species (H. antarcticus 0.16-0.23%.d-1, L. pholis, 0.31-0.76%.d-1), while liver (H. antarcticus 0.24-0.27%.d-1, L. pholis, 0.44-1.03%.d-1) and GIT were unaffected by temperature in H. antarcticus but increased non-linearly in L.pholis (H. antarcticus 0.22-0.26%.d-1, L. pholis, 0.40-0.86%.d-1). RNA to protein ratios were unaffected by temperature in H. antarcticus but increased weakly, in L.pholis WM and liver. In L.pholis, RNA translational efficiency increased significantly with temperature in all tissues, but only in liver in H. antarcticus. At the overlapping temperature of 3 °C, protein synthesis (WM 26%, Liver, 39%, GIT, 35%) and RNA translational efficiency (WM 273%, Liver, 271%, GIT, 300%) were significantly lower in H. antarcticus than L.pholis, while RNA to protein ratios were significantly higher (WM 270%, Liver 170%, GIT 186%). Tissue specific effects of temperature are detectable in both species. This study provides the first evidence, that tissue protein synthesis rates are constrained in Antarctic fishes.
摘要：
温度对组织蛋白合成速率的影响在温带和热带,但不是南极鱼。先前的研究通常表明，与温带亲戚相比，南极鱼类的生长速率较低，蛋白质周转水平较高。这项研究调查了低温如何影响极地鱼类物种的组织蛋白质合成和组织生长。南极群体，南极和温带的Harpagifer，Lipophryspholis，适应一系列重叠的水温，并在白肌肉（WM）中测量蛋白质合成，肝脏和胃肠道（GIT）。两种物种的WM蛋白合成速率均随温度线性增加（H。南极0.16-0.23%。d-1，L.pholis，0.31-0.76%。d-1),而肝脏(H.南极0.24-0.27%。d-1，L.pholis，0.44-1.03%。d-1）和GIT在南极H。受温度影响，但在L。pholis（H。南极0.22-0.26%。d-1，L.pholis，0.40-0.86%。d-1)。在南极嗜血杆菌中，RNA与蛋白质的比率不受温度的影响，但微弱地增加，在L.pholisWM和肝脏中。在L.pholis，在所有组织中，RNA翻译效率随温度的升高而显着增加，但只在南极H.在3°C的重叠温度下，蛋白质合成(WM26%，肝脏,39%,GIT,35%)和RNA翻译效率(WM273%，肝脏,271%,GIT,300%)显著低于南方H.pholis，而RNA与蛋白质的比率明显更高(WM为270%，肝脏170％，GIT186%）。温度的组织特异性效应在两个物种中均可检测到。这项研究提供了第一个证据，南极鱼类的组织蛋白质合成速率受到限制。
