Abstract：
Micromeria biflora (M.B) Benth has proven anti-inflammatory efficacy, thereby, the goal of the current investigation was to assess the anti-arthritic potential of M.B ethanolic extract and fractions as well as to investigate the likely mechanism of action. The effectiveness of M.B against acute arthritic manifestations was assessed using an arthritic model prompted by formaldehyde, whereas a chronic model was developed using an adjuvant called Complete Freund\'s in Sprague-Dawley rats. Weekly evaluations were conducted for parameters involving paw volume, body weight, and arthritic score; at the completion of the CFA model, hematological, biochemical and oxidative stress parameters as well as the level of various mediators (PGE2, IL-1β, TNFα, IL6, MMP2, 3, 9, VEGF, NF-ĸB, IL-10, and IL-4) were evaluated. The results demonstrated the plant\'s ability to treat arthritis by showing a significant decrease in paw volume, arthritic score, and histological characteristics. The levels of NF-ĸB, MMP2, 3, 9, IL6, IL1β, TNFα, and VEGF were all significantly reduced after treatment with plant extract and fractions. Plant extract and its fractions substantially preserved body weight loss, oxidative stress markers and levels of IL-4 and 1L-10. PGE2 levels were also shown to be reduced in the treatment groups, supporting the M.B immunomodulatory ability. Hematological and biochemical indicators were also normalized after M.B administration. Outcomes of the study validated the anti-arthritic and immunomodulatory attributes of M.B probably through modulating oxidative stress, inflammatory, pro-inflammatory and anti-inflammatory biomarkers.
摘要：
微生物（M.B)Benth已被证明具有抗炎功效，因此，本研究的目的是评估M.B乙醇提取物和组分的抗关节炎潜能,并研究可能的作用机制.使用甲醛提示的关节炎模型评估M.B对急性关节炎表现的有效性，而在Sprague-Dawley大鼠中使用称为完全弗氏佐剂的慢性模型。每周对涉及爪子体积的参数进行评估，体重,和关节炎评分；在CFA模型完成后，血液学,生化和氧化应激参数以及各种介质的水平(PGE2，IL-1β，TNFα,IL6，MMP2，3，9，VEGF，NF-B，评估IL-10和IL-4）。结果表明，该植物通过显示爪子体积的显著减少来治疗关节炎的能力，关节炎评分,和组织学特征。NF-B的水平，MMP2,3,9,IL6,IL1β,TNFα,用植物提取物和组分处理后，VEGF均显着降低。植物提取物及其部分基本上保存了体重减轻，氧化应激标志物和IL-4和1L-10的水平。PGE2水平也显示在治疗组中降低，支持M.B免疫调节能力。M.B给药后血液学和生化指标也恢复正常。研究结果验证了M.B的抗关节炎和免疫调节属性可能是通过调节氧化应激，炎症，促炎和抗炎生物标志物。
