Abstract：
BACKGROUND: It is reported that CD123 + HLA-DR- cells in PBMC are basophils, and CD203c, CD63, and FcεRI molecules are activation markers of basophils. However, little is known of CD123 + HLA-DR-cells in blood granulocytes.
OBJECTIVE: To investigate the presence of CD123 + HLA-DR- cells in the blood granulocytes and peripheral PBMC of patients with allergic rhinitis (AR), as well as the impact of allergens on the cell membrane markers of basophils.
METHODS: Flow cytometry was used to detect the expression of the membrane molecules.
RESULTS: While CD123 + HLA-DR- PBMCs are representative of basophils, their presence did not significantly change in patients with AR. In contrast, both the percentage and number of CD123 + HLA-DR- granulocytes, which make up only up to 50% of basophils, were significantly increased in patients with seasonal (sAR) and perennial AR (pAR). CD63+, CD203c+, and FcεRIα+ cells within CD123 + HLA-DR- granulocytes also showed enhanced activity in patients with AR. Allergen extracts from house dust mite allergen extract (HDME) and Artemisia sieversiana wild extract further increased the number of CD123 + HLA-DR- cells in granulocytes of sAR and pAR patients, as well as in PBMCs of pAR patients.
CONCLUSIONS: The use of CD123 + HLA-DR- granulocytes and PBMC may not be sufficient for diagnosing AR. Allergens could potentially contribute to the development of AR by influencing the number of CD123 + HLA-DR- cells, as well as the expression of CD63, CD203c, and FcεRIαin these cells.
OBJECTIVE: To investigate the presence of CD123 + HLA-DR- cells in the blood granulocytes and peripheral PBMC of patients with allergic rhinitis (AR), as well as the impact of allergens on the cell membrane markers of basophils.
METHODS: Flow cytometry was used to detect the expression of the membrane molecules.
RESULTS: While CD123 + HLA-DR- PBMCs are representative of basophils, their presence did not significantly change in patients with AR. In contrast, both the percentage and number of CD123 + HLA-DR- granulocytes, which make up only up to 50% of basophils, were significantly increased in patients with seasonal (sAR) and perennial AR (pAR). CD63+, CD203c+, and FcεRIα+ cells within CD123 + HLA-DR- granulocytes also showed enhanced activity in patients with AR. Allergen extracts from house dust mite allergen extract (HDME) and Artemisia sieversiana wild extract further increased the number of CD123 + HLA-DR- cells in granulocytes of sAR and pAR patients, as well as in PBMCs of pAR patients.
CONCLUSIONS: The use of CD123 + HLA-DR- granulocytes and PBMC may not be sufficient for diagnosing AR. Allergens could potentially contribute to the development of AR by influencing the number of CD123 + HLA-DR- cells, as well as the expression of CD63, CD203c, and FcεRIαin these cells.
摘要：
背景：据报道，PBMC中的CD123HLA-DR-细胞是嗜碱性粒细胞，和CD203c，CD63和FcεRI分子是嗜碱性粒细胞的活化标记。然而,血液粒细胞中的CD123+HLA-DR细胞鲜为人知。
目的：探讨变应性鼻炎（AR）患者外周血粒细胞和外周血PBMC中CD123+HLA-DR-细胞的存在，以及过敏原对嗜碱性粒细胞细胞膜标志物的影响。
方法：流式细胞术检测膜分子的表达。
结果：虽然CD123+HLA-DR-PBMC是嗜碱性粒细胞的代表，他们的存在在AR患者中没有显著变化.相比之下,CD123+HLA-DR-粒细胞的百分比和数量，只占50%的嗜碱性粒细胞，季节性（sAR）和常年性AR（pAR）患者均显着增加。CD63+,CD203c+,在AR患者中，CD123HLA-DR-粒细胞内的FcεRIα细胞也显示出增强的活性。来自屋尘螨变应原提取物(HDME)和青蒿野生提取物的变应原提取物进一步增加了sAR和pAR患者粒细胞中CD123+HLA-DR-细胞的数量,以及pAR患者的PBMC。
结论：使用CD123+HLA-DR-粒细胞和PBMC可能不足以诊断AR。变应原可能通过影响CD123+HLA-DR-细胞的数量来促进AR的发展，以及CD63，CD203c的表达，和FcεRIα在这些细胞中。
目的：探讨变应性鼻炎（AR）患者外周血粒细胞和外周血PBMC中CD123+HLA-DR-细胞的存在，以及过敏原对嗜碱性粒细胞细胞膜标志物的影响。
方法：流式细胞术检测膜分子的表达。
结果：虽然CD123+HLA-DR-PBMC是嗜碱性粒细胞的代表，他们的存在在AR患者中没有显著变化.相比之下,CD123+HLA-DR-粒细胞的百分比和数量，只占50%的嗜碱性粒细胞，季节性（sAR）和常年性AR（pAR）患者均显着增加。CD63+,CD203c+,在AR患者中，CD123HLA-DR-粒细胞内的FcεRIα细胞也显示出增强的活性。来自屋尘螨变应原提取物(HDME)和青蒿野生提取物的变应原提取物进一步增加了sAR和pAR患者粒细胞中CD123+HLA-DR-细胞的数量,以及pAR患者的PBMC。
结论：使用CD123+HLA-DR-粒细胞和PBMC可能不足以诊断AR。变应原可能通过影响CD123+HLA-DR-细胞的数量来促进AR的发展，以及CD63，CD203c的表达，和FcεRIα在这些细胞中。
