{Reference Type}: Journal Article
{Title}: Increased CD123 + HLA-DR- Granulocytes in Allergic Rhinitis and Influence of Allergens on Expression of Cell Membrane Markers.
{Author}: Xie H;Zhang H;Chen D;Cheng L;Gu F;Wang S;Liu M;Li L;Zeng Q;He S;
{Journal}: Am J Rhinol Allergy
{Volume}: 0
{Issue}: 0
{Year}: 2024 May 7
{Factor}: 2.3
{DOI}: 10.1177/19458924241252456
{Abstract}: <B>BACKGROUND: </B>It is reported that CD123 + HLA-DR- cells in PBMC are basophils, and CD203c, CD63, and FcεRI molecules are activation markers of basophils. However, little is known of CD123 + HLA-DR-cells in blood granulocytes.<BR><B>OBJECTIVE: </B>To investigate the presence of CD123 + HLA-DR- cells in the blood granulocytes and peripheral PBMC of patients with allergic rhinitis (AR), as well as the impact of allergens on the cell membrane markers of basophils.<BR><B>METHODS: </B>Flow cytometry was used to detect the expression of the membrane molecules.<BR><B>RESULTS: </B>While CD123 + HLA-DR- PBMCs are representative of basophils, their presence did not significantly change in patients with AR. In contrast, both the percentage and number of CD123 + HLA-DR- granulocytes, which make up only up to 50% of basophils, were significantly increased in patients with seasonal (sAR) and perennial AR (pAR). CD63+, CD203c+, and FcεRIα+ cells within CD123 + HLA-DR- granulocytes also showed enhanced activity in patients with AR. Allergen extracts from house dust mite allergen extract (HDME) and Artemisia sieversiana wild extract further increased the number of CD123 + HLA-DR- cells in granulocytes of sAR and pAR patients, as well as in PBMCs of pAR patients.<BR><B>CONCLUSIONS: </B>The use of CD123 + HLA-DR- granulocytes and PBMC may not be sufficient for diagnosing AR. Allergens could potentially contribute to the development of AR by influencing the number of CD123 + HLA-DR- cells, as well as the expression of CD63, CD203c, and FcεRIαin these cells.