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Abstract：
Schisandra henryi is an endemic species of medicinal potential known from traditional Chinese medicine. As part of this study, a complex biotechnological and phytochemical assessment was conducted on S. henryi with a focus on phenolic compounds and antioxidant profiling. The following in vitro cultures were tested: microshoot agar and callus, microshoot agitated, and suspension, along with the microshoot culture in PlantForm bioreactors. Qualitative profiling was performed by ultra-high-performance liquid chromatography with a photodiode array detector coupled with ion-trap mass spectrophotometry with electrospray ionization and then quantitative analysis by high-performance liquid chromatography with a diode array detector using standards. In the extracts, mainly the compounds from procyanidins were identified as well as phenolic acids (neochlorogenic acid, caffeic acid, protocatechuic acid) and catechin. The highest content of phenolic compounds was found for in vitro agar microshoot culture (max. total content 229.87 mg/100 g DW) and agitated culture (max. total content 22.82 mg/100 g DW). The max. TPC measured using the Folin-Ciocalteu assay was equal to 1240.51 mg GAE/100 g DW (agar microshoot culture). The extracts were evaluated for their antioxidant potential by the DPPH, FRAP, and chelate iron ion assays. The highest potential was indicated for agar microshoot culture (90% of inhibition and 59.31 nM/L TEAC, respectively). The research conducted on the polyphenol profiling and antioxidant potential of S. henryi in vitro culture extracts indicates the high therapeutic potential of this species. KEY POINTS: • Different types of S. henryi in vitro cultures were compared for the first time. • The S. henryi in vitro culture strong antioxidant potential was determined for the first time. • The polyphenol profiling of different types of S. henryi in vitro cultures was shown.
摘要：
北五味子是传统中药已知的具有潜在药用潜力的特有物种。作为这项研究的一部分,对S.henryi进行了复杂的生物技术和植物化学评估，重点是酚类化合物和抗氧化剂分析。测试了以下体外培养物：微芽琼脂和愈伤组织，微芽搅拌，和暂停，以及PlantForm生物反应器中的微枝培养。通过具有光电二极管阵列检测器的超高效液相色谱法以及具有电喷雾电离的离子阱质量分光光度法进行定性分析，然后通过具有二极管阵列检测器的高效液相色谱法使用标准品进行定量分析。在摘录中，主要鉴定了原花青素的化合物以及酚酸(新绿原酸，咖啡酸,原儿茶酸）和儿茶素。在体外琼脂微芽培养中发现酚类化合物的含量最高（最大。总含量229.87毫克/100克DW）和搅拌培养（最大总含量22.82毫克/100克DW)。max。使用Folin-Ciocalteu测定法测量的TPC等于1240.51mgGAE/100gDW(琼脂微芽培养物)。通过DPPH评估提取物的抗氧化潜力，FRAP,和螯合铁离子测定。琼脂微芽培养的潜力最高(90%的抑制和59.31nM/LTEAC,分别)。对S.henryi体外培养提取物的多酚分析和抗氧化潜力进行的研究表明，该物种具有很高的治疗潜力。关键点:•首次比较不同类型的Henryi体外培养物。•第一次确定了Henryi体外培养的强抗氧化潜力。•显示了不同类型的Henryi体外培养物的多酚分布。
