Abstract：
Two novel actinobacteria, designated as LP05-1T and LP11T, were isolated from the lichen Pyxine cocoes (Sw.) Nyl. collected in Bangkok, Thailand. Genotypic and phenotypic analyses revealed that both strains represented members of the genus Streptomyces. The 16S rRNA gene of LP05-1T showed the highest similarity to the genome of Streptomyces gelaticus (98.41 %), while the 16S rRNA gene of LP11T was most similar to that of Streptomyces cinerochromogenes (98.93 %). The major menaquinones in LP05-1T were MK-9(H8), MK-9(H6), MK-9(H4) and MK-9(H2), and in LP11T, they were MK-9(H8) and MK-9(H6). Both strains exhibited the major fatty acids iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0, with LP05-1T also possessing iso-C17 : 0. The polar lipids of LP05-1T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified lipid, while those of LP11T consisted of phosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified aminolipid and an unidentified glycolipid. The digital DNA-DNA hybridisation (dDDH) and average nucleotide identity (ANI) values indicated that both strains are distinct from each other with values below 70 and 95 %, respectively. dDDH, ANI by blast (ANIb) and ANI by MUMmer (ANIm) values between LP05-1T and its closely related type strains were 26.07-26.80 %, 81.24-82.01 % and 86.82-86.96 %, respectively, while those for LP11T and its closely related type strains were 30.70-31.70 %, 84.09-85.31 % and 88.02-88.39 %, respectively. The results of the taxonomic investigation, including dDDH and ANI values, indicate that LP05-1T and LP11T are novel type strains of two novel species within the genus Streptomyces. The names proposed are Streptomyces pyxinae sp. nov. for strain LP05-1T (=TBRC 15494T, =NBRC 115434T) and Streptomyces pyxinicus sp. nov. for strain LP11T (=TBRC 15493T, =NBRC 115421T).
摘要：
两种新型放线菌，指定为LP05-1T和LP11T，从地衣Pyxine茧中分离出来（Sw。）尼尔。收集在曼谷，泰国。基因型和表型分析表明，这两个菌株都代表链霉菌属的成员。LP05-1T的16SrRNA基因与凝胶链霉菌基因组相似性最高(98.41%),而LP11T的16SrRNA基因与嗜肉链霉菌最相似（98.93％）。LP05-1T中主要的甲萘醌是MK-9（H8），MK-9(H6),MK-9(H4)和MK-9(H2)，在LP11T中，它们是MK-9(H8)和MK-9(H6)。两种菌株均表现出主要的脂肪酸异C15：0，anteiso-C15：0，iso-C16：0和anteiso-C17：0，LP05-1T也具有iso-C17：0。LP05-1T的极性脂质包括磷脂酰乙醇胺，磷脂酰甘油，二磷脂酰甘油，磷脂酰肌醇,磷脂酰肌醇甘露糖苷和一种未知的脂质，而LP11T由磷脂酰乙醇胺组成，溶血磷脂酰乙醇胺，磷脂酰甘油，二磷脂酰甘油，磷脂酰肌醇,磷脂酰肌醇甘露糖苷,一种身份不明的氨基脂质和一种身份不明的糖脂.数字DNA-DNA杂交（dDDH）和平均核苷酸同一性（ANI）值表明两种菌株彼此不同，值低于70％和95％。分别。dDDH,LP05-1T及其密切相关型菌株之间的爆炸ANI（ANIb）和MUMmerANI（ANIm）值分别为26.07-26.80％，81.24-82.01%和86.82-86.96%，分别,而LP11T及其密切相关型菌株的比例为30.70-31.70%，84.09-85.31%和88.02-88.39%，分别。分类调查的结果，包括DDDH和ANI值，表明LP05-1T和LP11T是链霉菌属中两个新物种的新类型菌株。建议的名称是pyxinae链霉菌。11月。对于应变LP05-1T（=TBRC15494T，=NBRC115434T）和pyxinicus链霉菌。11月。对于应变LP11T（=TBRC15493T，=NBRC115421T)。
