Abstract：
This study presented the detection and quantification of capsular polysaccharide (CPS) as a biomarker for the diagnosis of melioidosis. After successfully screening four monoclonal antibodies (mAbs) previously determined to bind CPS molecules, the team developed a portable electrochemical immunosensor based on antibody-antigen interactions. The biosensor was able to detect CPS with a wide detection range from 0.1pg/mL to 1 μg/mL. The developed biosensor achieved high sensitivity for the detection of CPS spiked into both urine and serum. The developed assay platform was successfully programmed into a Windows app, and the sensor performance was evaluated with different spiked concentrations. The rapid electro-analytical device (READ) sensor showed great unprecedented sensitivity for the detection of CPS molecules in both serum and urine, and results were cross-validated with ELISA methods.
摘要：
这项研究提出了荚膜多糖（CPS）的检测和定量作为一种生物标志物，用于诊断类lioidosis。在成功筛选了先前确定与CPS分子结合的四种单克隆抗体（mAb）后，该团队开发了一种基于抗体-抗原相互作用的便携式电化学免疫传感器。生物传感器能够以0.1pg/mL至1μg/mL的宽检测范围检测CPS。开发的生物传感器对于检测掺入尿液和血清中的CPS具有很高的灵敏度。开发的检测平台已成功编程到Windows应用程序中，并在不同的加标浓度下评估了传感器的性能。快速电分析装置（READ）传感器对检测血清和尿液中的CPS分子显示出前所未有的灵敏度，结果与ELISA方法交叉验证。
