%0 Journal Article
%T Electrochemical Immunoassay for Capturing Capsular Polysaccharide of Burkholderia pseudomallei: Early Onsite Detection of Melioidosis.
%A Madhurantakam S
%A Karnam JB
%A Dhamu VN
%A Seetaraman S
%A Gates-Hollingsworth MA
%A AuCoin DP
%A Clark DV
%A Schully KL
%A Muthukumar S
%A Prasad S
%J ACS Infect Dis
%V 10
%N 6
%D 2024 Jun 14
%M 38712884
%F 5.578
%R 10.1021/acsinfecdis.4c00133
%X This study presented the detection and quantification of capsular polysaccharide (CPS) as a biomarker for the diagnosis of melioidosis. After successfully screening four monoclonal antibodies (mAbs) previously determined to bind CPS molecules, the team developed a portable electrochemical immunosensor based on antibody-antigen interactions. The biosensor was able to detect CPS with a wide detection range from 0.1pg/mL to 1 μg/mL. The developed biosensor achieved high sensitivity for the detection of CPS spiked into both urine and serum. The developed assay platform was successfully programmed into a Windows app, and the sensor performance was evaluated with different spiked concentrations. The rapid electro-analytical device (READ) sensor showed great unprecedented sensitivity for the detection of CPS molecules in both serum and urine, and results were cross-validated with ELISA methods.