{Reference Type}: Journal Article
{Title}: Electrochemical Immunoassay for Capturing Capsular Polysaccharide of Burkholderia pseudomallei: Early Onsite Detection of Melioidosis.
{Author}: Madhurantakam S;Karnam JB;Dhamu VN;Seetaraman S;Gates-Hollingsworth MA;AuCoin DP;Clark DV;Schully KL;Muthukumar S;Prasad S;
{Journal}: ACS Infect Dis
{Volume}: 10
{Issue}: 6
{Year}: 2024 Jun 14
{Factor}: 5.578
{DOI}: 10.1021/acsinfecdis.4c00133
{Abstract}: This study presented the detection and quantification of capsular polysaccharide (CPS) as a biomarker for the diagnosis of melioidosis. After successfully screening four monoclonal antibodies (mAbs) previously determined to bind CPS molecules, the team developed a portable electrochemical immunosensor based on antibody-antigen interactions. The biosensor was able to detect CPS with a wide detection range from 0.1pg/mL to 1 μg/mL. The developed biosensor achieved high sensitivity for the detection of CPS spiked into both urine and serum. The developed assay platform was successfully programmed into a Windows app, and the sensor performance was evaluated with different spiked concentrations. The rapid electro-analytical device (READ) sensor showed great unprecedented sensitivity for the detection of CPS molecules in both serum and urine, and results were cross-validated with ELISA methods.