PDF(Pubmed)
Abstract：
BACKGROUND: Mayaro virus (MAYV) is an emerging alphavirus, primarily transmitted by the mosquito Haemagogus janthinomys in Central and South America. However, recent studies have shown that Aedes aegypti, Aedes albopictus and various Anopheles mosquitoes can also transmit the virus under laboratory conditions. MAYV causes sporadic outbreaks across the South American region, particularly in areas near forests. Recently, cases have been reported in European and North American travelers returning from endemic areas, raising concerns about potential introductions into new regions. This study aims to assess the vector competence of three potential vectors for MAYV present in Europe.
METHODS: Aedes albopictus from Italy, Anopheles atroparvus from Spain and Culex pipiens biotype molestus from Belgium were exposed to MAYV and maintained under controlled environmental conditions. Saliva was collected through a salivation assay at 7 and 14 days post-infection (dpi), followed by vector dissection. Viral titers were determined using focus forming assays, and infection rates, dissemination rates, and transmission efficiency were calculated.
RESULTS: Results indicate that Ae. albopictus and An. atroparvus from Italy and Spain, respectively, are competent vectors for MAYV, with transmission possible starting from 7 dpi under laboratory conditions. In contrast, Cx. pipiens bioform molestus was unable to support MAYV infection, indicating its inability to contribute to the transmission cycle.
CONCLUSIONS: In the event of accidental MAYV introduction in European territories, autochthonous outbreaks could potentially be sustained by two European species: Ae. albopictus and An. atroparvus. Entomological surveillance should also consider certain Anopheles species when monitoring MAYV transmission.
METHODS: Aedes albopictus from Italy, Anopheles atroparvus from Spain and Culex pipiens biotype molestus from Belgium were exposed to MAYV and maintained under controlled environmental conditions. Saliva was collected through a salivation assay at 7 and 14 days post-infection (dpi), followed by vector dissection. Viral titers were determined using focus forming assays, and infection rates, dissemination rates, and transmission efficiency were calculated.
RESULTS: Results indicate that Ae. albopictus and An. atroparvus from Italy and Spain, respectively, are competent vectors for MAYV, with transmission possible starting from 7 dpi under laboratory conditions. In contrast, Cx. pipiens bioform molestus was unable to support MAYV infection, indicating its inability to contribute to the transmission cycle.
CONCLUSIONS: In the event of accidental MAYV introduction in European territories, autochthonous outbreaks could potentially be sustained by two European species: Ae. albopictus and An. atroparvus. Entomological surveillance should also consider certain Anopheles species when monitoring MAYV transmission.
摘要：
背景：Mayaro病毒（MAYV）是一种新兴的甲病毒，主要由中美洲和南美洲的蚊子Haemagogusjanthinomys传播。然而,最近的研究表明埃及伊蚊，白纹伊蚊和各种按蚊也可以在实验室条件下传播病毒。MAYV在整个南美地区造成了零星的爆发，特别是在森林附近的地区。最近,从流行地区返回的欧洲和北美旅行者报告了病例，引起人们对可能引入新地区的担忧。本研究旨在评估欧洲存在的MAYV的三种潜在载体的载体能力。
方法：意大利白纹伊蚊，来自西班牙的按蚊和来自比利时的淡色库蚊生物型molestus暴露于MAYV并保持在受控的环境条件下。在感染后7天和14天（dpi）通过唾液测定收集唾液，然后是矢量解剖。使用焦点形成测定法测定病毒滴度，和感染率，传播率，并计算了传输效率。
结果：结果表明Ae.白纹和安.来自意大利和西班牙的atroparvus,分别,是MAYV的有效载体，与传输可能从7dpi在实验室条件下开始。相比之下,Cx.Pipiensbioformmolestus无法支持MAYV感染，表明它无法为传输周期做出贡献。
结论：如果在欧洲领土上意外引入MAYV，本地爆发可能由两个欧洲物种维持：Ae。白纹和安.atroparvus.在监测MAYV传播时，昆虫学监测还应考虑某些按蚊物种。
方法：意大利白纹伊蚊，来自西班牙的按蚊和来自比利时的淡色库蚊生物型molestus暴露于MAYV并保持在受控的环境条件下。在感染后7天和14天（dpi）通过唾液测定收集唾液，然后是矢量解剖。使用焦点形成测定法测定病毒滴度，和感染率，传播率，并计算了传输效率。
结果：结果表明Ae.白纹和安.来自意大利和西班牙的atroparvus,分别,是MAYV的有效载体，与传输可能从7dpi在实验室条件下开始。相比之下,Cx.Pipiensbioformmolestus无法支持MAYV感染，表明它无法为传输周期做出贡献。
结论：如果在欧洲领土上意外引入MAYV，本地爆发可能由两个欧洲物种维持：Ae。白纹和安.atroparvus.在监测MAYV传播时，昆虫学监测还应考虑某些按蚊物种。
