METHODS: Aedes albopictus from Italy, Anopheles atroparvus from Spain and Culex pipiens biotype molestus from Belgium were exposed to MAYV and maintained under controlled environmental conditions. Saliva was collected through a salivation assay at 7 and 14 days post-infection (dpi), followed by vector dissection. Viral titers were determined using focus forming assays, and infection rates, dissemination rates, and transmission efficiency were calculated.
RESULTS: Results indicate that Ae. albopictus and An. atroparvus from Italy and Spain, respectively, are competent vectors for MAYV, with transmission possible starting from 7 dpi under laboratory conditions. In contrast, Cx. pipiens bioform molestus was unable to support MAYV infection, indicating its inability to contribute to the transmission cycle.
CONCLUSIONS: In the event of accidental MAYV introduction in European territories, autochthonous outbreaks could potentially be sustained by two European species: Ae. albopictus and An. atroparvus. Entomological surveillance should also consider certain Anopheles species when monitoring MAYV transmission.
方法:意大利白纹伊蚊,来自西班牙的按蚊和来自比利时的淡色库蚊生物型molestus暴露于MAYV并保持在受控的环境条件下。在感染后7天和14天(dpi)通过唾液测定收集唾液,然后是矢量解剖。使用焦点形成测定法测定病毒滴度,和感染率,传播率,并计算了传输效率。
结果:结果表明Ae.白纹和安.来自意大利和西班牙的atroparvus,分别,是MAYV的有效载体,与传输可能从7dpi在实验室条件下开始。相比之下,Cx.Pipiensbioformmolestus无法支持MAYV感染,表明它无法为传输周期做出贡献。
结论:如果在欧洲领土上意外引入MAYV,本地爆发可能由两个欧洲物种维持:Ae。白纹和安.atroparvus.在监测MAYV传播时,昆虫学监测还应考虑某些按蚊物种。