Abstract：
Long noncoding RNAs (lncRNAs) are regulatory RNAs. Saccharomyces cerevisiae strains transcribe hundreds of lncRNAs. LncRNAs can regulate the expression of adjacent genes (cis-regulation) or distant genes from lncRNAs (trans-regulation). Here, we analyzed the potential global cis and trans-regulation of lncRNAs of yeast subjected to ethanol stress. For potential cis regulation, for BMA641-A and S288C strains, we observed that most lncRNA-neighbor gene pairs increased the expression at a certain point followed by a decrease, and vice versa. Based on the transcriptome profile and triple helix prediction between lncRNAs and promoters of coding genes, we observed nine different ways of potential trans regulation that work in a strain-specific manner. Our data provide an initial landscape of potential cis and trans regulation in yeast, which seems to be strain-specific.
摘要：
长链非编码RNA(lncRNA)是调节性RNA。酿酒酵母菌株转录数百个lncRNA。LncRNA可以调节邻近基因(顺式调节)或来自lncRNA的远端基因(反式调节)的表达。这里,我们分析了受到乙醇胁迫的酵母lncRNAs的潜在全局顺式和反式调控。对于潜在的顺式调节，对于BMA641-A和S288C菌株，我们观察到大多数lncRNA-邻居基因对在某个点增加表达，然后减少，反之亦然。基于lncRNAs和编码基因启动子之间的转录组谱和三螺旋预测，我们观察到了9种不同的潜在反式调节方式，这些方式以菌株特异性方式起作用。我们的数据提供了酵母中潜在的顺式和反式调节的初始景观，这似乎是菌株特有的。
