Abstract：
Deep-sea hydrothermal vents host archaeal and bacterial thermophilic communities, including taxonomically and functionally diverse Thermoproteota. Despite their prevalence in high-temperature submarine communities, Thermoproteota are chronically under-represented in genomic databases and issues have emerged regarding their nomenclature, particularly within the Aeropyrum-Thermodiscus-Caldisphaera. To resolve some of these problems, we identified 47 metagenome-assembled genomes (MAGs) within this clade, from 20 previously published deep-sea hydrothermal vent and submarine volcano metagenomes, and 24 MAGs from public databases. Using phylogenomic analysis, Genome Taxonomy Database Toolkit (GTDB-Tk) taxonomic assessment, 16S rRNA gene phylogeny, average amino acid identity (AAI) and functional gene patterns, we re-evaluated of the taxonomy of the Aeropyrum-Thermodiscus-Caldisphaera. At least nine genus-level clades were identified with two or more MAGs. In accordance with SeqCode requirements and recommendations, we propose names for three novel genera, viz. Tiamatella incendiivivens, Hestiella acidicharens and Calypsonella navitae. A fourth genus was also identified related to Thermodiscus maritimus, for which no available sequenced genome exists. We propose the novel species Thermodiscus eudorianus to describe our high-quality Thermodiscus MAG, which represents the type genome for the genus. All three novel genera and T. eudorianus are likely anaerobic heterotrophs, capable of fermenting protein-rich carbon sources, while some Tiamatella, Calypsonella and T. eudorianus may also reduce polysulfides, thiosulfate, sulfur and/or selenite, and the likely acidophile, Hestiella, may reduce nitrate and/or perchlorate. Based on phylogenomic evidence, we also propose the family Acidilobaceae be amended to include Caldisphaera, Aeropyrum, Thermodiscus and Stetteria and the novel genera described here.
摘要：
深海热液喷口拥有古细菌和细菌嗜热群落，包括分类学和功能上不同的Thermoproteota。尽管它们在高温潜艇社区普遍存在，Thermoproteota在基因组数据库中长期代表性不足，并且出现了有关其命名法的问题，特别是在Aeropyrum-Thermodiscus-Caldisphaera中。为了解决其中一些问题，我们在这个进化枝中确定了47个宏基因组组装的基因组（MAG），从20个先前发表的深海热液喷口和海底火山宏基因组中，和来自公共数据库的24个MAG。使用系统基因组分析，基因组分类数据库工具包(GTDB-Tk)分类评估，16SrRNA基因系统发育，平均氨基酸同一性(AAI)和功能基因模式，我们重新评估了Aeropyrum-Thermodiscus-Caldisphaera的分类学。用两个或更多个MAG鉴定出至少9个属级进化枝。根据SeqCode的要求和建议，我们提出了三个新属的名字，viz.Tiamatella感染,酸尾藻和纳维卡利氏球菌。还确定了与Thermodiscusmaritimus有关的第四属，没有可用的测序基因组存在。我们提出了新物种Thermodiscuseudorianus来描述我们的高质量ThermodiscusMAG，代表该属的类型基因组。所有三个新属和T.eudorianus都可能是厌氧异养生物，能够发酵富含蛋白质的碳源，而一些Tiamatella，Calypsonella和T.eudorianus也可以减少多硫化物，硫代硫酸盐，硫和/或亚硒酸盐，和可能的嗜酸者，赫斯特拉,可以减少硝酸盐和/或高氯酸盐。根据系统发育学证据,我们还建议对酸性科进行修改，以包括Caldisphaera，Aeropyrum,Thermodiscus和Stetteria以及此处描述的新属。
