四个嗜盐古细菌菌株,BCD28T,BND7T,PSR21T,和PSRA2T,从沿海和内陆盐渍土壤中分离出来,分别。这四个菌株和目前的Halomarina物种之间的16SrRNA和rpoB基因序列相似性分别为95.9-96.6%和86.9-90.3%,分别。系统发育和系统基因组分析显示,这四个菌株与Halomarina属的当前物种紧密聚集。AAI,ANI,这四个菌株和目前的Halomarina物种中的dDDH值为65.3-68.4%,75.8-77.7%,20.3-22.0%,分别,明显低于物种划分的阈值。菌株BCD28T,BND7T,PSR21T,根据不同表型特征的比较,可以将PSRA2T与当前的Halomarina物种区分开。这四个菌株的主要极性脂质是磷脂酰甘油(PG),磷脂酰甘油磷酸甲酯(PGP-Me),和四到五种糖脂。仅在菌株BND7T中检测到磷脂酰甘油硫酸酯(PGS)。表型,系统发育,和基于基因组的分析表明,菌株BCD28T(=CGMCC1.18776T=JCM34908T),BND7T(=CGMCC1.18778T=JCM34910T),PSR21T(=CGMCC1.17027T=JCM34147T),和PSRA2T(=CGMCC1.17214T=JCM34148T)代表了Halomarina属的四个新物种,名字为Halomarinalitoreasp。11月。,Halomarinapelagicasp.11月。,HalomarinaHalobiasp.11月。,和Halomarinaordinariasp.11月。是提议的。
Four halophilic archaeal strains, BCD28T, BND7T, PSR21T, and PSRA2T, were isolated from coastal and inland saline soil, respectively. The 16S rRNA and rpoB\' gene sequence similarities among these four strains and current species of Halomarina were 95.9-96.6% and 86.9-90.3%, respectively. Phylogenetic and phylogenomic analyses revealed that these four strains tightly cluster with the current species of the genus Halomarina. The AAI, ANI, and dDDH values among these four strains and current species of Halomarina were 65.3-68.4%, 75.8-77.7%, and 20.3-22.0%, respectively, clearly below the threshold values for species demarcation. Strains BCD28T, BND7T, PSR21T, and PSRA2T could be differentiated from the current species of Halomarina based on the comparison of diverse phenotypic characteristics. The major polar lipids of these four strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), and four to five glycolipids. Phosphatidylglycerol sulfate (PGS) was only detected in strain BND7T. The phenotypic, phylogenetic, and genome-based analyses suggested that strains BCD28T (= CGMCC 1.18776T = JCM 34908T), BND7T (= CGMCC 1.18778T = JCM 34910T), PSR21T (= CGMCC 1.17027T = JCM 34147T), and PSRA2T (= CGMCC 1.17214T = JCM 34148T) represent four novel species of the genus Halomarina, for which the names Halomarina litorea sp. nov., Halomarina pelagica sp. nov., Halomarina halobia sp. nov., and Halomarina ordinaria sp. nov. are proposed.