嗜温,嗜酸性古细菌,菌株M1T,是从Vulcano岛的岩石样本中分离出来的,意大利。该生物的细胞是平均直径为1μm的球菌。一些细胞拥有细丝。该菌株在15至52°C和pH0.5-4.0的温度范围内生长,在40°C和pH1.0时生长最佳。菌株M1T是需氧和化学有机营养的,在复杂的基质上生长,如酪蛋白氨基酸,胰蛋白酶,胰蛋白胨,酵母和牛肉提取物。以元素硫或还原硫化合物的氧化为代价没有增长,黄铁矿,或观察到硫酸亚铁。核心脂质是具有0至4个环戊烷部分和古菌醇的甘油二二苯酰甘油四醚脂质(跨膜),有微量的羟基古细菌.主要的醌是MK-7:7。M1T基因组大小为1.67Mbp,G+C含量为39.76mol%,这两个特征都在热等离子体的共同范围内。基于16SrRNA基因序列的系统发育分析将菌株M1T置于具有90.9、90.3和90.5%序列同一性的热质序列中,与具有有效公布名称的生物体中最接近的SSUrRNA基因序列,嗜酸性热等离子体,热等离子火山和嗜热单胞菌,分别。根据我们基因组的结果,系统发育,生理和化学分类学研究,我们认为菌株M1T(=DSM116605T=JCM36570T)代表了一个新的属和种,氧等离子体子午线。11月。,sp.11月。,在热等离子体订单内。
A mesophilic, hyperacidophilic archaeon, strain M1T, was isolated from a rock sample from Vulcano Island, Italy. Cells of this organism were cocci with an average diameter of 1 µm. Some cells possessed filaments. The strain grew in the range of temperatures between 15 and 52 °C and pH 0.5-4.0 with growth optima at 40 °C and pH 1.0. Strain M1T was aerobic and chemoorganotrophic, growing on complex substrates, such as casamino acids, trypticase, tryptone, yeast and beef extracts. No growth at expenses of oxidation of elemental sulphur or reduced sulphur compounds, pyrite, or ferrous sulphate was observed. The core lipids were glycerol dibiphytanyl glycerol tetraether lipids (membrane spanning) with 0 to 4 cyclopentane moieties and archaeol, with trace amounts of hydroxy archaeol. The dominant quinone was MK-7 : 7. The genome size of M1T was 1.67 Mbp with a G+C content of 39.76 mol%, and both characteristics were well within the common range for Thermoplasmatales. The phylogenetic analysis based on 16S rRNA gene sequence placed the strain M1T within the order Thermoplasmatales with sequence identities of 90.9, 90.3 and 90.5% to the closest SSU rRNA gene sequences from organisms with validly published names, Thermoplasma acidophilum, Thermoplasma volcanium and Thermogymnomonas acidicola, respectively. Based on the results of our genomic, phylogenetic, physiological and chemotaxonomic studies, we propose that strain M1T (=DSM 116605T=JCM 36570T) represents a new genus and species, Oxyplasma meridianum gen. nov., sp. nov., within the order Thermoplasmatales.