Abstract：
UNASSIGNED: The incremental biological changes in the synovial microenvironment of the shoulder in acute and chronic instability that may contribute to joint degeneration are poorly understood. Proteomic analysis of synovial fluid in patients with shoulder instability may improve our understanding of proteins that are shed into shoulder synovial fluid after an injury.
UNASSIGNED: Injury-specific factors such as the direction of instability and the severity of glenoid and humeral bone loss are associated with the proteome of synovial fluid in patients with shoulder instability.
UNASSIGNED: Descriptive laboratory study.
UNASSIGNED: Synovial fluid lavage samples were compared between patients with anterior (n = 12) and posterior (n = 8) instability and those without instability (n = 5). Synovial proteins were identified with liquid chromatography-tandem mass spectrometry. Orthogonal validation of protein targets found to be significant on tandem mass spectrometry was performed in a separate set of prospective patients with Western blotting. Data were processed and analyzed, and P values were adjusted with the Benjamini-Hochberg method for multiple comparisons.
UNASSIGNED: A total of 25 patients were included. Tandem mass spectrometry identified 720 protein groups in synovial fluid of patients with shoulder instability. There were 4 synovial proteins that were significantly expressed in patients with anterior instability relative to posterior instability: periostin (POSTN) (adjusted P value = .03; log fold change [logFc] = 4.7), transforming growth factor beta-induced protein ig-h3 (adjusted P value = .05; logFc = 1.7), collagen type VI alpha-3 chain (adjusted P value = .04; logFc = 2.6), and coagulation factor V (adjusted P value = .04; logFc = -3.3). Among these targets, POSTN showed a moderate correlation with the Hill-Sachs lesion size (r = 0.7). Prospective validation with Western blotting confirmed a significantly higher level of POSTN in synovial fluid of patients with anterior instability (P = .00025; logFc = 5.1).
UNASSIGNED: Proteomic analysis enriched our understanding of proteins that were secreted into shoulder synovial fluid of patients with shoulder instability. The identification of POSTN, a proinflammatory catabolic protein involved with tissue remodeling and repair, as a significant target in anterior shoulder instability is a novel finding. Therefore, further study is warranted to determine the role that POSTN may play in the progression of bone loss and posttraumatic osteoarthritis.
UNASSIGNED: Proteomic analysis of synovial fluid in patients with shoulder instability improved our understanding of this abnormality after an injury.
UNASSIGNED: Injury-specific factors such as the direction of instability and the severity of glenoid and humeral bone loss are associated with the proteome of synovial fluid in patients with shoulder instability.
UNASSIGNED: Descriptive laboratory study.
UNASSIGNED: Synovial fluid lavage samples were compared between patients with anterior (n = 12) and posterior (n = 8) instability and those without instability (n = 5). Synovial proteins were identified with liquid chromatography-tandem mass spectrometry. Orthogonal validation of protein targets found to be significant on tandem mass spectrometry was performed in a separate set of prospective patients with Western blotting. Data were processed and analyzed, and P values were adjusted with the Benjamini-Hochberg method for multiple comparisons.
UNASSIGNED: A total of 25 patients were included. Tandem mass spectrometry identified 720 protein groups in synovial fluid of patients with shoulder instability. There were 4 synovial proteins that were significantly expressed in patients with anterior instability relative to posterior instability: periostin (POSTN) (adjusted P value = .03; log fold change [logFc] = 4.7), transforming growth factor beta-induced protein ig-h3 (adjusted P value = .05; logFc = 1.7), collagen type VI alpha-3 chain (adjusted P value = .04; logFc = 2.6), and coagulation factor V (adjusted P value = .04; logFc = -3.3). Among these targets, POSTN showed a moderate correlation with the Hill-Sachs lesion size (r = 0.7). Prospective validation with Western blotting confirmed a significantly higher level of POSTN in synovial fluid of patients with anterior instability (P = .00025; logFc = 5.1).
UNASSIGNED: Proteomic analysis enriched our understanding of proteins that were secreted into shoulder synovial fluid of patients with shoulder instability. The identification of POSTN, a proinflammatory catabolic protein involved with tissue remodeling and repair, as a significant target in anterior shoulder instability is a novel finding. Therefore, further study is warranted to determine the role that POSTN may play in the progression of bone loss and posttraumatic osteoarthritis.
UNASSIGNED: Proteomic analysis of synovial fluid in patients with shoulder instability improved our understanding of this abnormality after an injury.
摘要：
■对可能导致关节变性的急性和慢性不稳定中肩部滑膜微环境的增量生物学变化知之甚少。肩关节不稳患者滑液的蛋白质组学分析可以提高我们对损伤后脱落到肩关节滑液中的蛋白质的理解。
■损伤特异性因素，例如不稳定的方向以及关节盂和肱骨骨丢失的严重程度，与肩关节不稳患者的滑液蛋白质组有关。
■描述性实验室研究。
■在前（n=12）和后（n=8）不稳定的患者和没有不稳定的患者（n=5）之间比较了滑液灌洗样本。用液相色谱-串联质谱法鉴定滑膜蛋白。在一组单独的具有蛋白质印迹的前瞻性患者中进行在串联质谱上发现显著的蛋白质靶标的正交验证。对数据进行了处理和分析,用Benjamini-Hochberg方法调整P值进行多重比较。
■共纳入25例患者。串联质谱鉴定了肩关节不稳患者滑液中的720个蛋白质组。相对于后部不稳定，前部不稳定患者有4种滑膜蛋白显着表达：骨膜蛋白（POSTN）（调整后的P值=.03；对数倍数变化[logFc]=4.7），转化生长因子β诱导的蛋白Ig-h3（调整后的P值=0.05；logFc=1.7），胶原蛋白VI型α-3链（调整后的P值=.04；logFc=2.6），和凝血因子V（调整后的P值=.04；logFc=-3.3）。在这些目标中,POSTN与Hill-Sachs病变大小呈中等相关性（r=0.7）。Western印迹的前瞻性验证证实，前部不稳定患者的滑液中POSTN水平显着升高（P=.00025；logFc=5.1）。
蛋白质组学分析丰富了我们对肩关节不稳患者肩关节滑液中分泌的蛋白质的理解。POSTN的识别，一种与组织重塑和修复有关的促炎分解代谢蛋白，作为前肩关节不稳定的重要目标是一个新的发现。因此,有必要进一步研究以确定POSTN在骨丢失和创伤后骨关节炎进展中的作用.
■肩关节不稳患者滑液的蛋白质组学分析提高了我们对损伤后这种异常的理解。
■损伤特异性因素，例如不稳定的方向以及关节盂和肱骨骨丢失的严重程度，与肩关节不稳患者的滑液蛋白质组有关。
■描述性实验室研究。
■在前（n=12）和后（n=8）不稳定的患者和没有不稳定的患者（n=5）之间比较了滑液灌洗样本。用液相色谱-串联质谱法鉴定滑膜蛋白。在一组单独的具有蛋白质印迹的前瞻性患者中进行在串联质谱上发现显著的蛋白质靶标的正交验证。对数据进行了处理和分析,用Benjamini-Hochberg方法调整P值进行多重比较。
■共纳入25例患者。串联质谱鉴定了肩关节不稳患者滑液中的720个蛋白质组。相对于后部不稳定，前部不稳定患者有4种滑膜蛋白显着表达：骨膜蛋白（POSTN）（调整后的P值=.03；对数倍数变化[logFc]=4.7），转化生长因子β诱导的蛋白Ig-h3（调整后的P值=0.05；logFc=1.7），胶原蛋白VI型α-3链（调整后的P值=.04；logFc=2.6），和凝血因子V（调整后的P值=.04；logFc=-3.3）。在这些目标中,POSTN与Hill-Sachs病变大小呈中等相关性（r=0.7）。Western印迹的前瞻性验证证实，前部不稳定患者的滑液中POSTN水平显着升高（P=.00025；logFc=5.1）。
蛋白质组学分析丰富了我们对肩关节不稳患者肩关节滑液中分泌的蛋白质的理解。POSTN的识别，一种与组织重塑和修复有关的促炎分解代谢蛋白，作为前肩关节不稳定的重要目标是一个新的发现。因此,有必要进一步研究以确定POSTN在骨丢失和创伤后骨关节炎进展中的作用.
■肩关节不稳患者滑液的蛋白质组学分析提高了我们对损伤后这种异常的理解。
