Abstract：
METHODS: Tocomonoenols (T1) are little-known vitamin E derivatives naturally occurring in foods. Limited knowledge exists regarding the cellular uptake and metabolism of α-tocomonoenol (αT1) and none about that of γ-tocomonoenol (γT1).
RESULTS: The study investigates the cytotoxicity, uptake, and metabolism of αT1 and γT1 in HepG2 cells compared to the α- and γ-tocopherols (T) and -tocotrienols (T3). None of the studied tocochromanols are cytotoxic up to 100 µmol L-1. The uptake of the γ-congeners is significantly higher than that of the corresponding α-forms, whereas no significant differences are observed based on the degree of saturation of the sidechain. Carboxymethylbutyl-hydroxychromans (CMBHC) are the predominant short-chain metabolites of all tocochromanols and conversion is higher for γT1 than αT1 as well as for the γ-congeners of T and T3. The rate of metabolism increases with the number of double bonds in the sidechain. The rate of metabolic conversion of the T1 is more similar to tocopherols than to that of the tocotrienols.
CONCLUSIONS: This is the first evidence that both αT1 and γT1 follow the same sidechain degradation pathway and exert similar rates of metabolism than tocopherols. Therefore, investigation into the biological activities of tocomonoenols is warranted.
RESULTS: The study investigates the cytotoxicity, uptake, and metabolism of αT1 and γT1 in HepG2 cells compared to the α- and γ-tocopherols (T) and -tocotrienols (T3). None of the studied tocochromanols are cytotoxic up to 100 µmol L-1. The uptake of the γ-congeners is significantly higher than that of the corresponding α-forms, whereas no significant differences are observed based on the degree of saturation of the sidechain. Carboxymethylbutyl-hydroxychromans (CMBHC) are the predominant short-chain metabolites of all tocochromanols and conversion is higher for γT1 than αT1 as well as for the γ-congeners of T and T3. The rate of metabolism increases with the number of double bonds in the sidechain. The rate of metabolic conversion of the T1 is more similar to tocopherols than to that of the tocotrienols.
CONCLUSIONS: This is the first evidence that both αT1 and γT1 follow the same sidechain degradation pathway and exert similar rates of metabolism than tocopherols. Therefore, investigation into the biological activities of tocomonoenols is warranted.
摘要：
方法：Tocomonoenols（T1）是鲜为人知的天然存在于食品中的维生素E衍生物。关于α-生育烯醇（αT1）的细胞摄取和代谢的知识有限，而关于γ-生育烯醇（γT1）的知识却一无所知。
结果：该研究调查了细胞毒性，摄取，与α-和γ-生育酚（T）和-生育三烯酚（T3）相比，HepG2细胞中αT1和γT1的代谢。所研究的生育色酚在100µmolL-1下都没有细胞毒性。γ-同源物的摄取明显高于相应的α-形式，而根据侧链的饱和度没有观察到显着差异。羧甲基丁基-羟基色满（CMBHC）是所有生育色满醇的主要短链代谢物，γT1的转化率高于αT1以及T和T3的γ同源物。代谢速率随着侧链中双键的数量而增加。与生育三烯酚相比，T1的代谢转化率更类似于生育酚。
结论：这是第一个证据，表明αT1和γT1遵循相同的侧链降解途径，并发挥与生育酚相似的代谢率。因此,有必要对妥莫诺醇的生物活性进行研究。
结果：该研究调查了细胞毒性，摄取，与α-和γ-生育酚（T）和-生育三烯酚（T3）相比，HepG2细胞中αT1和γT1的代谢。所研究的生育色酚在100µmolL-1下都没有细胞毒性。γ-同源物的摄取明显高于相应的α-形式，而根据侧链的饱和度没有观察到显着差异。羧甲基丁基-羟基色满（CMBHC）是所有生育色满醇的主要短链代谢物，γT1的转化率高于αT1以及T和T3的γ同源物。代谢速率随着侧链中双键的数量而增加。与生育三烯酚相比，T1的代谢转化率更类似于生育酚。
结论：这是第一个证据，表明αT1和γT1遵循相同的侧链降解途径，并发挥与生育酚相似的代谢率。因此,有必要对妥莫诺醇的生物活性进行研究。
