Abstract：
In mammals, IL-22 is considered as a critical cytokine regulating of immunity and homeostasis at barrier surfaces. Although IL-22 have been functional characterization in different species of fish, the studies about distinct responses of IL-22 in different organs/tissues/cell types is rather limited. Here, we identified and cloned IL-22 gene (named as Ec-IL-22) from grouper (Epinephelus coioides). Ec-IL-22 gene was detected in all orangs/tissues examined, and was induced in intestine, gill, spleen, head kidney, and primary head kidney/intestine leukocytes following the stimulation of LPS and poly (I:C), as well as Vibrio harveyi and Singapore grouper iridovirus infection (SGIV). In addition, the stimulation of DSS could induce the expression of Ec-IL-22 in intestine and primary leukocytes from intestine. Importantly, the treatment of recombinant Ec-IL-22 induced the mRNA level of proinflammatory cytokines in primary intestine/head kidney leukocytes. The present results improve the understanding of expression patterns and functional characteristics of fish IL-22 in different organs/tissues/cell types.
摘要：
在哺乳动物中,IL-22被认为是调节屏障表面的免疫和稳态的关键细胞因子。尽管IL-22在不同种类的鱼类中具有功能特性，关于IL-22在不同器官/组织/细胞类型中的不同反应的研究相当有限。这里,我们从石斑鱼（Epinepheluscoioides）中鉴定并克隆了IL-22基因（命名为Ec-IL-22）。Ec-IL-22基因在所有检查的orangs/组织中检测到，在肠道中诱导，吉尔,脾,脾头肾,和LPS和聚（I：C）刺激后的原发性头肾/肠白细胞，以及哈维氏弧菌和新加坡石斑鱼虹彩病毒感染（SGIV）。此外,刺激DSS可以诱导Ec-IL-22在肠道和肠道原代白细胞的表达。重要的是,重组Ec-IL-22的治疗诱导了原发性肠/头肾白细胞中促炎细胞因子的mRNA水平。本结果提高了对鱼IL-22在不同器官/组织/细胞类型中的表达模式和功能特征的理解。
