Abstract：
Timely detection of early-stage cancer holds immense potential in enhancing prognostic outcomes. There is an increasing desire for versatile tools to enable simple, sensitive, and cost-effective cancer detection. By exploiting the extraintestinal metabolic inertness and efficiency renal clearance of sucrose, we designed a liposome nanosensor using sucrose as a messenger to convert tumor-specific esterase activity into glucose meter readout, enabling economical and sensitive urinalysis for cancer detection in point-of-care testing (POCT). Our results demonstrate that the nanosensors exhibited significant signal differences between tumor-bearing and healthy mice in both orthotopic and metastatic tumor models. Additionally, efficient elimination of the nanosensors through the hepatobiliary pathway was observed with no significant toxicity. Such a non-invasive diagnostic modality significantly assists in personalized pharmacological treatment and follow-up efficacy assessment. We envision that this modular liposome nanosensor platform might be applied for economically detecting diverse diseases via a simple urinary test.
摘要：
及时检测早期癌症在增强预后结果方面具有巨大潜力。人们越来越希望使用多功能工具来实现简单、敏感,和具有成本效益的癌症检测。通过利用蔗糖的肠外代谢惰性和肾脏清除率，我们设计了一种脂质体纳米传感器，使用蔗糖作为信使，将肿瘤特异性酯酶活性转化为葡萄糖计读数，在即时检测（POCT）中实现对癌症检测的经济和灵敏的分析。我们的结果表明，在原位和转移性肿瘤模型中，纳米传感器在荷瘤小鼠和健康小鼠之间表现出明显的信号差异。广告上，通过肝胆途径观察到纳米传感器的有效消除，没有明显的毒性。这种非侵入性诊断方式显著有助于个性化的药物治疗和后续低疗效评估。我们设想这种模块化脂质体纳米传感器平台可用于通过简单的尿检经济地检测各种疾病。
