Abstract：
The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) (Lepidoptera, Noctuidae), is a highly polyphagous invasive pest that damages various crops. Pesticide control is the most common and effective strategy to control FAW. In this study, we evaluated the toxicity of metaflumizone and indoxacarb against third-instar FAW larvae using the insecticide-incorporated artificial diet method under laboratory conditions. Both metaflumizone and indoxacarb exhibited substantial toxicity against FAW, with LC50 values of 2.43 and 14.66 mg/L at 72 h, respectively. The sublethal effects of metaflumizone and indoxacarb on parental and F1 generation FAW were investigated by exposing third-instar larvae to LC10 and LC30 concentrations of these insecticides. Sublethal exposure to these two insecticides significantly shortened adult longevity, extended pupal developmental times and led to reduced pupal weight, pupation rates, and adult fecundity in the treated parental generation and F1 generation at LC10 or LC30 concentrations, in comparison to the control group. The larval developmental times were shortened in the parental generation but prolonged in the F1 generation, after being treated with sublethal concentrations of metaflumizone. Furthermore, larvae exposed to LC10 or LC30 concentrations of indoxacarb exhibited elevated activity levels of cytochrome P450 monooxygenase and glutathione S-transferase, which coincides with the observed synergistic effect of piperonyl butoxide and diethyl maleate. In conclusion, the high toxicity and negative impact of metaflumizone and indoxacarb on FAW provided significant implications for the rational utilization of insecticides against this pest.
摘要：
秋季粘虫（一汽），节食夜蛾（J.E.Smith）（鳞翅目，夜蛾科)，是一种高度多食的入侵害虫，会损害各种作物。农药控制是一汽最常见和最有效的控制策略。在这项研究中,我们在实验室条件下使用掺入杀虫剂的人工饮食方法评估了甲氟虫胺和indoxacarb对三龄一汽幼虫的毒性。甲氟利嗪和吲哚沙卡威对一汽均表现出明显的毒性，72h时LC50值为2.43和14.66mg/L，分别。通过将三龄幼虫暴露于LC10和LC30浓度的这些杀虫剂中，研究了甲氟虫胺和吲哚沙卡威对父母和F1代FAW的亚致死作用。这两种杀虫剂的亚致死性暴露显著缩短了成人的寿命,延长p的发育时间，并导致p的重量减少，化蛹率,在LC10或LC30浓度下，处理的亲本世代和F1世代的成虫繁殖力，与对照组相比。幼虫发育时间在亲代缩短，但在F1代延长，用亚致死浓度的甲氟利胺治疗后。此外，暴露于LC10或LC30浓度的吲哚沙卡威的幼虫表现出细胞色素P450单加氧酶和谷胱甘肽S-转移酶的活性水平升高，这与观察到的胡椒基丁醚和马来酸二乙酯的协同作用一致。总之,甲氟虫胺和吲哚沙卡威对一汽的高毒性和负面影响为合理利用杀虫剂防治该害虫提供了重要意义。
