Abstract：
The Ussing chamber is a tool for analyzing drug absorption. We investigated whether the Ussing chamber can be used to analyze the process from digestion to absorption of protein in the gastrointestinal tract. Mixtures containing infant formula, whole cow\'s milk, processed soy milk, enteral nutrition, or human breast milk, were placed in the apical membrane side equipped with Caco-2 cells. After the addition of first pepsin then pancreatin, samples from the apical and basal membranes were collected. Infant formula showed the highest digestibility and absorption rate. This may be attributed to the presence of whey protein, which is rapidly digested and absorbed. The digestion and absorption of human breast milk showed different results in each donor, suggesting that digestion and absorption may vary among individuals. We concluded that the Ussing chamber can continuously analyze the process from digestion to absorption of proteins in the gastrointestinal tract.
摘要：
Ussing室是用于分析药物吸收的工具。我们调查了Ussing室是否可用于分析胃肠道中蛋白质从消化到吸收的过程。含有婴儿配方食品的混合物，全牛奶，加工豆浆,肠内营养，或者人类母乳，置于配备Caco-2细胞的顶膜侧。添加第一个胃蛋白酶，然后添加胰酶后，收集根尖和基底膜的样品。婴儿配方奶粉的消化率和吸收率最高。这可能归因于乳清蛋白的存在，迅速消化和吸收。人母乳的消化吸收在每个供体中表现出不同的结果，这表明消化和吸收可能因个体而异。我们得出的结论是，Ussing室可以连续分析胃肠道中蛋白质从消化到吸收的过程。
