Abstract：
As the aging process accelerates, the age structure of blood donors turns to older and even aged groups. Methylmalonic acid (MMA), a byproduct of propionate metabolism, may be upregulated in the serum of older adults. As a mediator of chronic disease and tumor progression, the MMA content in blood products has become the focus of research. Absolute concentrations of MMA in blood products were determined based on the liquid chromatography-tandem mass spectrometry, so as to analyze how they were affected by donors\' age, sex, and frequency of blood donation. The MMA content in leukocyte-depleted suspended red blood cell (lds-RBC) was significantly higher than that in fresh plasma (p<0.0001). The MMA content among five age groups showed no difference in either fresh plasma or lds-RBCs. The MMA content in fresh plasma was similar in the parameters of the sex, whereas that in lds-RBCs was higher in males than that in females (p=0.035). There were no significant differences in MMA content when it comes to different frequencies of blood donors in either fresh plasma or lds-RBCs. Additionally, there was no significant difference or clear trend in the rate of elevated plasma MMA levels among different sexes, age groups, and blood donation frequency groups. MMA in the blood products from donors in China does not compromise the safety of blood transfusions for cancer patients. Nevertheless, there is a need to focus on MMA levels in Chinese and to develop race-specific and age-specific normal reference ranges.
摘要：
随着老化过程的加速，献血者的年龄结构转向老年甚至老年群体。甲基丙二酸(MMA),丙酸代谢的副产品，可能在老年人的血清中上调。作为慢性疾病和肿瘤进展的介质，血液制品中MMA的含量已成为研究的重点。基于液相色谱-串联质谱法测定血液制品中MMA的绝对浓度。以便分析他们如何受到捐赠者年龄的影响，性别,献血的频率。白细胞耗尽的悬浮红细胞(lds-RBC)中的MMA含量显著高于新鲜血浆中的MMA含量(p<0.0001)。五个年龄组的MMA含量在新鲜血浆或lds-RBC中均无差异。新鲜血浆中的MMA含量在性别参数上相似，而lds-RBC中男性高于女性(p=0.035)。当涉及新鲜血浆或lds-RBC中不同频率的献血者时，MMA含量没有显着差异。此外,血浆MMA水平升高率在不同性别间无显著差异或明显趋势,年龄组,和献血频率组。中国献血者血液制品中的MMA不会影响癌症患者输血的安全性。然而，有必要关注中国人的MMA水平，并制定特定种族和特定年龄的正常参考范围。
