PDF(Pubmed)
Abstract：
Carbapenem-resistant Acinetobacter spp. is associated with nosocomial infections in intensive care unit patients, resulting in high mortality. Although Acinetobacter spp. represent a serious public health problem worldwide, there are a few studies related to the presence of carbapenemases in health care facilities and other environmental settings in Ecuador. The main aim of this study was to characterize the carbapenem-resistant Acinetobacter spp. isolates obtained from four hospitals (52) and from five rivers (27) close to Quito. We used the disc diffusion and EDTA sinergy tests to determine the antimicrobial susceptibility and the production of metallo β-lactamases, respectively. We carried out a multiplex PCR of gyrB gene and the sequencing of partial rpoB gene to bacterial species identification. We performed molecular screening of nine carbapenem-resistant genes (blaSPM, blaSIM, blaGIM, blaGES, blaOXA-23, blaOXA-24, blaOXA-51, blaOXA-58, and blaOXA-143) by multiplex PCR, followed by identification using sequencing of blaOXA genes. Our findings showed that carbapenem-resistant A. baumannii were the main species found in health care facilities and rivers. Most of the clinical isolates came from respiratory tract samples and harbored blaOXA-23, blaOXA-366, blaOXA-72, blaOXA-65, blaOXA-70, and blaOXA-143-like genes. The river isolates harbored only the blaOXA-51 and probably blaOXA-259 genes. We concluded that the most predominant type of carbapenem genes among isolates were both blaOXA-23 and blaOXA-65 among A. baumannii clinical isolates.
摘要：
耐碳青霉烯类不动杆菌。与重症监护病房患者的医院感染有关，导致高死亡率。虽然不动杆菌属。代表了世界范围内严重的公共卫生问题，在厄瓜多尔,有一些关于卫生保健设施和其他环境环境中存在碳青霉烯酶的研究.这项研究的主要目的是表征耐碳青霉烯的不动杆菌属。从四家医院（52）和靠近基多的五条河流（27）获得的分离株。我们使用圆盘扩散和EDTAsinergy测试来确定抗菌药物敏感性和金属β-内酰胺酶的产生，分别。我们进行了gyrB基因的多重PCR和部分rpoB基因的测序以鉴定细菌种类。我们对9个碳青霉烯类耐药基因进行了分子筛选(blaSPM，blaSIM,BlaGIM,blages,blaOXA-23，blaOXA-24，blaOXA-51，blaOXA-58和blaOXA-143）通过多重PCR，然后使用blaOXA基因测序进行鉴定。我们的发现表明，耐碳青霉烯类鲍曼不动杆菌是在医疗机构和河流中发现的主要物种。大多数临床分离株来自呼吸道样本，并带有blaOXA-23，blaOXA-366，blaOXA-72，blaOXA-65，blaOXA-70和blaOXA-143样基因。河流分离物仅包含blaOXA-51和可能的blaOXA-259基因。我们得出的结论是，在鲍曼不动杆菌临床分离株中，碳青霉烯基因的最主要类型是blaOXA-23和blaOXA-65。
