Abstract：
This study assessed the bacteriological quality of raw, treated, and distributed water from Ede-Erinle and Opa reservoirs in Osun State, Nigeria. This was to determine the potability of water from these waterwork stations. Eighteen sampling points were established across the two reservoir networks for this study. Samples were collected bi-monthly for two annual cycles. Serial dilution and pour plate methods were employed for the enumeration of bacterial load. Total heterotrophic bacteria count (THBC) and total coliform bacteria count (TCBC) were enumerated on nutrient and MacConkey agar at 37 °C, respectively. Bacterial isolates were characterized using biochemical identification methods with reference to Bergey\'s Manual of Determinative Bacteriology. Bacterial isolates and biofilm formation were further identified molecularly through the PCR method using specific universal primers. Mean values of THBC and TCBC in distributed water from Ede-Erinle (9.61 × 104 ± 1.50 × 104 CFU/mL; 69.56 ± 26.81 CFU/mL) and Opa waterworks (9.58 × 104 ± 2.55 × 104 CFU/mL; 142.94 ± 44.41 CFU/mL) exceeded permissible limits for drinking water. Paenibacillus lautus, Bacillus pseudomycoides, Pseudomonas aeruginosa, and Pseudomonas stutzeri showed biofilm-forming capacity. The study concluded that the presence of coliforms and biofilm-forming bacteria in distributed water implies that the water is unfit for consumption without further treatment.
摘要：
这项研究评估了原料的细菌学质量，治疗,并从奥孙州的埃德-埃林勒和奥帕水库分配水，尼日利亚。这是为了确定来自这些供水站的水的可使用性。本研究在两个储层网络上建立了18个采样点。每两个月收集一次样品，持续两个年度周期。采用连续稀释和倾倒板方法进行细菌负荷的计数。在37°C下，在营养物和MacConkey琼脂上对异养细菌总数（THBC）和大肠杆菌总数（TCBC）进行计数，分别。参考Bergey的《确定细菌学手册》，使用生化鉴定方法对细菌分离株进行了表征。使用特异性通用引物通过PCR方法进一步分子鉴定细菌分离物和生物膜形成。来自Ede-Erinle的分布式水中的THBC和TCBC的平均值（9.61×104±1.50×104CFU/mL；69.56±26.81CFU/mL）和Opa自来水厂（9.58×104±2.55×104CFU/mL；142.94±44.41CFU/mL）超过了饮用水的允许限值。月桂芽孢杆菌,假芽孢杆菌，铜绿假单胞菌,和Stutzeri假单胞菌显示生物膜形成能力。该研究得出的结论是，分布式水中大肠杆菌和形成生物膜的细菌的存在意味着水不适合在未经进一步处理的情况下食用。
