PDF(Pubmed)
Abstract：
The plant cell wall provides a strong yet flexible barrier to protect cells from the external environment. Modifications of the cell wall, either during development or under stress conditions, can induce cell wall integrity responses and ultimately lead to alterations in gene expression, hormone production, and cell wall composition. These changes in cell wall composition presumably require remodelling of the secretory pathway to facilitate synthesis and secretion of cell wall components and cell wall synthesis/remodelling enzymes from the Golgi apparatus. Here, we used a combination of live-cell confocal imaging and transmission electron microscopy to examine the short-term and constitutive impact of isoxaben, which reduces cellulose biosynthesis, and Driselase, a cocktail of cell-wall-degrading fungal enzymes, on cellular processes during cell wall integrity responses in Arabidopsis. We show that both treatments altered organelle morphology and triggered rebalancing of the secretory pathway to promote secretion while reducing endocytic trafficking. The actin cytoskeleton was less dynamic following cell wall modification, and organelle movement was reduced. These results demonstrate active remodelling of the endomembrane system and actin cytoskeleton following changes to the cell wall.
摘要：
植物细胞壁提供了一个强大而灵活的屏障来保护细胞免受外部环境的影响。细胞壁的修饰，无论是在发育过程中还是在压力条件下，可以诱导细胞壁完整性反应，并最终导致基因表达的改变，激素产生，和细胞壁组成。细胞壁组成的这些变化可能需要对分泌途径进行重塑，以促进细胞壁成分和细胞壁合成酶从高尔基体的合成和分泌。这里,我们使用了活细胞共聚焦成像和透射电子显微镜的组合，以检查短期和组成的影响，这减少了纤维素的生物合成，和driselase，一种细胞壁降解真菌酶的混合物，细胞壁完整性反应过程中的细胞过程。我们表明，两种治疗方法都改变了细胞器形态，并触发了分泌途径的重新平衡，以促进分泌，同时减少了内吞运输。细胞壁修饰后，肌动蛋白细胞骨架的动态性较低，细胞器运动减少。这些结果表明，随着细胞壁的变化，内膜系统和肌动蛋白细胞骨架的主动重塑。
