PDF(Pubmed)
Abstract：
Canine distemper virus (CDV) can cause fatal infections in giant pandas. Vaccination is crucial to prevent CDV infection in giant pandas. In this study, two bacterium-like particle vaccines F3-GEM and H4-GEM displaying the trimeric F protein or tetrameric H protein of CDV were constructed based on the Gram-positive enhanced-matrix protein anchor (GEM-PA) surface display system. Electron microscopy and Western blot results revealed that the F or H protein was successfully anchored on the surface of GEM particles. Furthermore, one more bacterium-like particle vaccine F3 and H4-GEM was also designed, a mixture consisting of F3-GEM and H4-GEM at a ratio of 1:1. To evaluate the effect of the three vaccines, mice were immunized with F3-GEM, H4-GEM or F3 and H4-GEM. It was found that the level of IgG-specific antibodies and neutralizing antibodies in the F3 and H4-GEM group was higher than the other two groups. Additionally, F3 and H4-GEM also increased the secretion of Th1-related and Th2-related cytokines. Moreover, F3 and H4-GEM induce IgG and neutralizing antibodies\' response in dogs. Conclusions: In summary, F3 and H4-GEM can provoke better immune responses to CDV in mice and dogs. The bacterium-like particle vaccine F3 and H4-GEM might be a potential vaccine candidate for giant pandas against CDV infection.
摘要：
犬瘟热病毒（CDV）可导致大熊猫致命感染。接种疫苗对预防大熊猫CDV感染至关重要。在这项研究中,基于革兰氏阳性增强基质蛋白锚（GEM-PA）表面展示系统，构建了两种显示CDV三聚体F蛋白或四聚体H蛋白的细菌样颗粒疫苗F3-GEM和H4-GEM。电子显微镜和Western印迹结果表明，F或H蛋白成功锚定在GEM颗粒表面。此外，还设计了一种类似细菌的颗粒疫苗F3和H4-GEM，由F3-GEM和H4-GEM以1:1的比例组成的混合物。为了评估三种疫苗的效果，用F3-GEM免疫小鼠，H4-GEM或F3和H4-GEM。发现F3和H4-GEM组的IgG特异性抗体和中和抗体水平高于其他两组。此外,F3和H4-GEM也增加了Th1相关和Th2相关细胞因子的分泌。此外，F3和H4-GEM在狗中诱导IgG和中和抗体应答。结论：总之，F3和H4-GEM可以在小鼠和狗中引起对CDV的更好的免疫应答。细菌样颗粒疫苗F3和H4-GEM可能是大熊猫抗CDV感染的潜在疫苗候选物。
