Abstract:
In traditional Chinese medicine, Aurantii Fructus Immatures (AFIs) have been utilized for more than 2000 years. The proportions of different fruit parts are crucial for evaluating AFI quality in China. However, the basis for this statement\'s substance is unclear. Differences in quality are intimately correlated with a plant\'s metabolite composition. On the basis of a widely targeted metabolome, this study intended to investigate the metabolite composition and evaluate the antioxidant capacity of the peel and pulp of an AFI. Metabolites were identified and quantified by UHPLC-QqQ-MS. To assess their antioxidant ability, DPPH and ABTS assays were carried out. There were 1327 chemical compounds identified by UHPLC-QqQ-MS. After screening the differential metabolites using a multivariate statistical analysis, it was found that there were 695 significant differences in the metabolites between the peel and the pulp. Among them, it was discovered that the content of active ingredients in the peel group was higher than that in the pulp group. Furthermore, the aqueous extracts from the peel showed stronger antioxidant capacities than those from the pulp. The metabolites and antioxidant capacities were significantly different between the peel and the pulp. This study of different fruit parts might provide a guide for AFI quality assessments.
摘要:
在中医中,AurantiiFrummatures(AFIs)已使用了2000多年。不同水果部分的比例对于评估中国AFI质量至关重要。然而,这一声明的实质依据尚不清楚。质量的差异与植物的代谢物组成密切相关。基于广泛靶向的代谢组,本研究旨在研究AFI果皮和果肉的代谢物组成并评估其抗氧化能力。通过UHPLC-QqQ-MS鉴定和定量代谢物。为了评估它们的抗氧化能力,进行DPPH和ABTS测定。通过UHPLC-QqQ-MS鉴定出1327种化合物。在使用多元统计分析筛选差异代谢物后,发现果皮和果肉之间的代谢物有695个显着差异。其中,发现果皮组的活性成分含量高于果肉组。此外,来自果皮的水提取物比来自果肉的水提取物显示出更强的抗氧化能力。果皮和果肉之间的代谢产物和抗氧化能力显着不同。这项对不同水果部位的研究可能为AFI质量评估提供指导。