PDF(Pubmed)
Abstract：
Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) is able to infect many economically important crops and thus causes substantial losses in the global agricultural economy. Pst DC3000 can be divided into virulent lines and avirulent lines. For instance, the pathogen effector avrRPM1 of avirulent line Pst-avrRpm1 (Pst DC3000 avrRpm1) can be recognized and detoxified by the plant. To further compare the pathogenicity mechanisms of virulent and avirulent Pst DC3000, a comprehensive analysis of the acetylome and succinylome in Arabidopsis thaliana was conducted following infection with virulent line Pst DC3000 and avirulent line Pst-avrRpm1. In this study, a total of 1625 acetylated proteins encompassing 3423 distinct acetylation sites were successfully identified. Additionally, 229 succinylated proteins with 527 unique succinylation sites were detected. A comparison of these modification profiles between plants infected with Pst DC3000 and Pst-avrRpm1 revealed significant differences. Specifically, modification sites demonstrated inconsistencies, with a variance of up to 10% compared to the control group. Moreover, lysine acetylation (Kac) and lysine succinylation (Ksu) displayed distinct preferences in their modification patterns. Lysine acetylation is observed to exhibit a tendency towards up-regulation in Arabidopsis infected with Pst-avrRpm1. Conversely, the disparity in the number of Ksu up-regulated and down-regulated sites was not as pronounced. Motif enrichment analysis disclosed that acetylation modification sequences are relatively conserved, and regions rich in polar acidic/basic and non-polar hydrophobic amino acids are hotspots for acetylation modifications. Functional enrichment analysis indicated that the differentially modified proteins are primarily enriched in the photosynthesis pathway, particularly in relation to light-capturing proteins. In conclusion, this study provides an insightful profile of the lysine acetylome and succinylome in A. thaliana infected with virulent and avirulent lines of Pst DC3000. Our findings revealed the potential impact of these post-translational modifications (PTMs) on the physiological functions of the host plant during pathogen infection. This study offers valuable insights into the complex interactions between plant pathogens and their hosts, laying the groundwork for future research on disease resistance and pathogenesis mechanisms.
摘要：
丁香假单胞菌pv。番茄DC3000（PstDC3000）能够感染许多经济上重要的作物，因此在全球农业经济中造成重大损失。PstDC3000可分为毒系和无毒系。例如,无毒系Pst-avrRpm1（PstDC3000avrRpm1）的病原体效应子avrRPM1可以被植物识别和解毒。为了进一步比较强毒系PstDC3000和无毒系Pst-avrRpm1感染拟南芥后，对拟南芥的乙酰和琥珀酰进行了全面分析。在这项研究中,总共1625个乙酰化蛋白,包括3423个不同的乙酰化位点被成功鉴定.此外,检测到具有527个独特琥珀酰化位点的229个琥珀酰化蛋白。用PstDC3000和Pst-avrRpm1感染的植物之间的这些修饰谱的比较揭示了显着差异。具体来说,修改站点显示不一致，与对照组相比，差异高达10%。此外，赖氨酸乙酰化（Kac）和赖氨酸琥珀酰化（Ksu）在其修饰模式中显示出不同的偏好。观察到赖氨酸乙酰化在感染Pst-avrRpm1的拟南芥中表现出上调的趋势。相反，Ksu上调和下调位点的数量差异并不明显.基序富集分析揭示乙酰化修饰序列相对保守,和富含极性酸性/碱性和非极性疏水氨基酸的区域是乙酰化修饰的热点。功能富集分析表明，差异修饰的蛋白质主要富集在光合作用途径中，特别是与光捕获蛋白有关。总之,这项研究提供了感染PstDC3000毒系和无毒系的拟南芥中赖氨酸乙酰组和琥珀酰的有见地的概况。我们的发现揭示了这些翻译后修饰（PTM）在病原体感染期间对宿主植物生理功能的潜在影响。这项研究为植物病原体与其宿主之间的复杂相互作用提供了有价值的见解，为今后研究抗病性和发病机制奠定基础。
