PDF(Pubmed)
Abstract：
The filamentous ascomycete Trichoderma reesei, known for its prolific cellulolytic enzyme production, recently also gained attention for its secondary metabolite synthesis. Both processes are intricately influenced by environmental factors like carbon source availability and light exposure. Here, we explore the role of the transcription factor STE12 in regulating metabolic pathways in T. reesei in terms of gene regulation, carbon source utilization and biosynthesis of secondary metabolites. We show that STE12 is involved in regulating cellulase gene expression and growth on carbon sources associated with iron homeostasis. STE12 impacts gene regulation in a light dependent manner on cellulose with modulation of several CAZyme encoding genes as well as genes involved in secondary metabolism. STE12 selectively influences the biosynthesis of the sorbicillinoid trichodimerol, while not affecting the biosynthesis of bisorbibutenolide, which was recently shown to be regulated by the MAPkinase pathway upstream of STE12 in the signaling cascade. We further report on the biosynthesis of dehydroacetic acid (DHAA) in T. reesei, a compound known for its antimicrobial properties, which is subject to regulation by STE12. We conclude, that STE12 exerts functions beyond development and hence contributes to balance the energy distribution between substrate consumption, reproduction and defense.
摘要：
丝状子囊里氏木霉，以其丰富的纤维素分解酶生产而闻名，最近也因其次生代谢产物的合成而受到关注。这两个过程都受到环境因素的复杂影响，例如碳源的可用性和光照。这里,我们从基因调控方面探讨了转录因子STE12在调节里氏木霉代谢途径中的作用,碳源利用和次生代谢产物的生物合成。我们表明，STE12参与调节纤维素酶基因的表达和与铁稳态相关的碳源的生长。STE12以光依赖的方式影响纤维素的基因调控，并调节几种CAZyme编码基因以及参与次生代谢的基因。STE12选择性地影响山梨糖醇的生物合成，而不影响生物合成的生物合成，最近显示受信号级联中STE12上游的MAP激酶途径调节。我们进一步报道了里氏木霉中脱氢乙酸（DHAA）的生物合成，一种以抗菌特性而闻名的化合物，受STE12的监管。我们得出结论，STE12发挥超出开发的功能，因此有助于平衡衬底消耗之间的能量分布，繁殖和防御。
