Abstract:
Elevated levels of superoxide anion radicals (O2·-) have been implicated in the pathogenesis of a variety of diseases, such as cancer, inflammatory diseases and autoimmune diseases. To determine the O2·- concentration for assisting disease detection, a method based on surface-enhanced Raman scattering (SERS) combined with transparent polymer microneedles has been developed. Photocrosslinked NOA61 is used to prepare microneedles with sulfhydryl group, which can contribute to anchor gold nanoparticles (Au NPs) functionalized by p-mercaptobenzoic acid (PATP). This work successfully constructed SERS microneedles for in situ detection. A REDOX reaction occurred between PATP and O2·-, resulting in the formation of dimethylaminoborane (DMAB) and a subsequent change in Raman signal. Based on the quantitative relationship between the change of peak area ratio at 1042 cm-1 and 1077 cm-1 and the concentration change of O2·-, a standard curve with a linear range of 0-480 ng/mL was constructed. The SERS microneedles were effectively employed to track melanoma progression in mice, establishing a fundamental correlation between O2·- concentration and melanoma stage, as confirmed by ELISA. The benefits of this approach, including convenience, in situ applicability, and low cost, are anticipated to offer novel insights for non-invasive in situ detection, potentially enhancing disease monitoring and diagnosis.
摘要:
超氧阴离子自由基(O2·-)水平的升高与多种疾病的发病机理有关,比如癌症,炎症性疾病和自身免疫性疾病。为了确定辅助疾病检测的O2·-浓度,开发了一种基于表面增强拉曼散射(SERS)与透明聚合物微针结合的方法。光交联的NOA61用于制备具有巯基的微针,这可以有助于锚定被对巯基苯甲酸(PATP)官能化的金纳米粒子(AuNPs)。这项工作成功地构建了用于原位检测的SERS微针。PATP和O2·-之间发生氧化还原反应,导致二甲基氨基硼烷(DMAB)的形成和拉曼信号的后续变化。根据1042cm-1和1077cm-1处的峰面积比变化与O2·-浓度变化之间的定量关系,构建线性范围为0-480ng/mL的标准曲线。SERS微针被有效地用于追踪小鼠的黑色素瘤进展,建立O2·-浓度与黑色素瘤分期之间的基本相关性,如通过ELISA确认的。这种方法的好处,包括便利性,原位适用性,成本低,预计将为非侵入性原位检测提供新的见解,有可能加强疾病监测和诊断。