PDF(Pubmed)
Abstract：
High blood cholesterol levels are a major risk factor for cardiovascular diseases. A purified aqueous extract of Fucus vesiculosus, rich in phlorotannins and peptides, has been described for its potential to inhibit cholesterol biosynthesis and intestinal absorption. In this work, the effect of this extract on intestinal cells\' metabolites and proteins was analysed to gain a deeper understanding of its mode of action on lipids\' metabolism, particularly concerning the absorption and transport of exogenous cholesterol. Caco-2 cells, differentiated into enterocytes, were exposed to the extract, and analysed by untargeted metabolomics and proteomics. The results of the metabolomic analysis showed statistically significant differences in glutathione content of cells exposed to the extract compared to control cells, along with an increased expression of fatty acid amides in exposed cells. A proteomic analysis showed an increased expression in cells exposed to the extract compared to control cells of FAB1 and NPC1, proteins known to be involved in lipid metabolism and transport. To the extent of our knowledge, this study is the first use of untargeted metabolomics and a proteomic analysis to investigate the effects of F. vesiculosus on differentiated Caco-2 cells, offering insights into the molecular mechanism of the extract\'s compounds on intestinal cells.
摘要：
高血胆固醇水平是心血管疾病的主要危险因素。一种纯化的水提物，富含间氯单宁和多肽，已经描述了其抑制胆固醇生物合成和肠道吸收的潜力。在这项工作中,分析了这种提取物对肠细胞代谢产物和蛋白质的影响，以更深入地了解其对脂质代谢的作用方式，特别是关于外源性胆固醇的吸收和运输。Caco-2细胞,分化为肠细胞，暴露于提取物中，并通过非靶向代谢组学和蛋白质组学进行分析。代谢组学分析结果显示，与对照细胞相比，暴露于提取物的细胞的谷胱甘肽含量存在统计学上的显着差异，随着暴露细胞中脂肪酸酰胺的表达增加。蛋白质组分析显示，与FAB1和NPC1的对照细胞相比，暴露于提取物的细胞中的表达增加，FAB1和NPC1是已知与脂质代谢和转运有关的蛋白质。在我们的知识范围内，这项研究是首次使用非靶向代谢组学和蛋白质组学分析来研究水痘对分化的Caco-2细胞的影响，提供对提取物化合物对肠细胞的分子机制的见解。
