PDF(Pubmed)
Abstract：
The early detection of procalcitonin (PCT) is crucial for diagnosing bacterial infections due to its high sensitivity and specificity. While colloidal gold colorimetric and immune-chemiluminescence methods are commonly employed in clinical detection, the former lacks sensitivity, and the latter faces challenges with a brief luminescence process and an elevated background. Here, we introduce a novel approach for the quantitative analysis of PCT using surface-enhanced Raman spectroscopy (SERS), leveraging the enhanced properties of metal nanoparticles. Simultaneously, we employed a magnetic nanoparticle coating and surface biofunctionalization modification to immobilize PCT-trapping antibodies, creating the required immune substrates. The resulting magnetic nanoparticles and antibody complexes, acting as carriers and recognition units, exhibited superparamagnetism and the specific recognition of biomarkers. Then, this complex efficiently underwent magnetic separation with an applied magnetic field, streamlining the cumbersome steps of traditional ELISA and significantly reducing the detection time. In conclusion, the exploration of immunomagnetic bead detection technology based on surface-enhanced Raman spectroscopy holds crucial practical significance for the sensitive detection of PCT.
摘要：
降钙素原（PCT）的早期检测由于其高灵敏度和特异性而对于诊断细菌感染至关重要。虽然胶体金比色法和免疫化学发光法通常用于临床检测，前者缺乏敏感性，后者面临着短暂的发光过程和升高的背景的挑战。这里,我们介绍了一种使用表面增强拉曼光谱（SERS）定量分析PCT的新方法，利用金属纳米粒子的增强特性。同时,我们采用磁性纳米粒子涂层和表面生物功能化修饰来固定PCT捕获抗体，创造所需的免疫底物。产生的磁性纳米粒子和抗体复合物，作为载体和识别单位，表现出超顺磁性和生物标志物的特异性识别。然后，这种复合物在外加磁场的作用下有效地进行了磁分离，简化了传统ELISA的繁琐步骤，显著缩短了检测时间。总之,基于表面增强拉曼光谱的免疫磁珠检测技术的探索对于PCT的灵敏检测具有重要的现实意义。
