PDF(Pubmed)
Abstract：
Fluctuating ocean conditions are rearranging whole networks of marine communities-from individual-level physiological thresholds to ecosystem function. Physiological studies support predictions from individual-level responses (biochemical, cellular, tissue, respiratory potential) based on laboratory experiments. The otolith-isotope method of recovering field metabolic rate has recently filled a gap for the bony fishes, linking otolith stable isotope composition to in situ oxygen consumption and experienced temperature estimates. Here, we review the otolith-isotope method focusing on the biochemical and physiological processes that yield estimates of field metabolic rate. We identify a multidisciplinary pathway in the application of this method, providing concrete research goals (field, modeling) aimed at linking individual-level physiological data to higher levels of biological organization. We hope that this review will provide researchers with a transdisciplinary \'roadmap\', guiding the use of the otolith-isotope method to bridge the gap between individual-level physiology, observational field studies, and modeling efforts, while ensuring that in situ data is central in marine policy-making aimed at mitigating climatic and anthropogenic threats.
摘要：
波动的海洋条件正在重新安排整个海洋社区网络-从个体水平的生理阈值到生态系统功能。生理研究支持来自个体水平反应的预测(生化，细胞，组织,呼吸电位)基于实验室实验。恢复田间代谢率的耳石同位素方法最近填补了硬骨鱼的空白，将耳石稳定同位素组成与原位耗氧量和经历的温度估计联系起来。这里,我们回顾了耳石同位素方法，重点是生化和生理过程，这些过程可以估算田间代谢率。我们确定了应用这种方法的多学科途径，提供具体的研究目标(领域，建模)旨在将个体水平的生理数据与更高层次的生物组织联系起来。我们希望这次审查将为研究人员提供一个跨学科的“路线图”，指导使用耳石同位素方法弥合个体生理学之间的差距，实地观察研究，和建模工作，同时确保现场数据在旨在减轻气候和人为威胁的海洋决策中至关重要。
