Abstract：
DEAD-box RNA helicases, a prominent subfamily within the RNA helicase superfamily 2 (SF2), play crucial roles in the growth, development, and abiotic stress responses of plants. This study identifies 146 DEAD-box RNA helicase genes (GhDEADs) and categorizes them into four Clades (Clade A-D) through phylogenetic analysis. Promoter analysis reveals cis-acting elements linked to plant responses to light, methyl jasmonate (MeJA), abscisic acid (ABA), low temperature, and drought. RNA-seq data demonstrate that Clade C GhDEADs exhibit elevated and ubiquitous expression across different tissues, validating their connection to leaf development through real-time quantitative polymerase chain reaction (RT-qPCR) analysis. Notably, over half of GhDEADs display up-regulation in the leaves of virus-induced gene silencing (VIGS) plants of GhVIR-A/D (members of m6A methyltransferase complex, which regulate leaf morphogenesis). In conclusion, this study offers a comprehensive insight into GhDEADs, emphasizing their potential involvement in leaf development.
摘要：
DEAD-boxRNA解旋酶,RNA解旋酶超家族2（SF2）中的一个突出的亚家族，在成长中发挥关键作用，发展,和植物的非生物胁迫响应。这项研究鉴定了146个DEAD-boxRNA解旋酶基因（GhDEAD），并通过系统发育分析将其分类为四个进化枝（进化枝A-D）。启动子分析揭示了与植物对光的反应相关的顺式作用元件，茉莉酸甲酯（MeJA），脱落酸（ABA），低温,和干旱。RNA-seq数据表明，CladeCGhDEAD在不同组织中表现出升高和普遍存在的表达，通过实时定量聚合酶链反应（RT-qPCR）分析验证它们与叶片发育的联系。值得注意的是,超过一半的GhDEAD在GhVIR-A/D的病毒诱导基因沉默（VIGS）植物的叶片中显示出上调（m6A甲基转移酶复合物的成员，调节叶片形态发生)。总之,这项研究提供了对GhDEAD的全面了解，强调他们在叶片发育中的潜在参与。
