{Reference Type}: Journal Article
{Title}: Genome-wide identification and analysis of DEAD-box RNA helicases in Gossypium hirsutum.
{Author}: Rao R;Huang X;Wang X;Li X;Liao H;Abuduwaili N;Wei X;Li D;Huang G;
{Journal}: Gene
{Volume}: 920
{Issue}: 0
{Year}: 2024 Aug 20
{Factor}: 3.913
{DOI}: 10.1016/j.gene.2024.148495
{Abstract}: DEAD-box RNA helicases, a prominent subfamily within the RNA helicase superfamily 2 (SF2), play crucial roles in the growth, development, and abiotic stress responses of plants. This study identifies 146 DEAD-box RNA helicase genes (GhDEADs) and categorizes them into four Clades (Clade A-D) through phylogenetic analysis. Promoter analysis reveals cis-acting elements linked to plant responses to light, methyl jasmonate (MeJA), abscisic acid (ABA), low temperature, and drought. RNA-seq data demonstrate that Clade C GhDEADs exhibit elevated and ubiquitous expression across different tissues, validating their connection to leaf development through real-time quantitative polymerase chain reaction (RT-qPCR) analysis. Notably, over half of GhDEADs display up-regulation in the leaves of virus-induced gene silencing (VIGS) plants of GhVIR-A/D (members of m6A methyltransferase complex, which regulate leaf morphogenesis). In conclusion, this study offers a comprehensive insight into GhDEADs, emphasizing their potential involvement in leaf development.