Abstract：
Recently there is much debate in the scientific community over the impact of the food matrix on prebiotic efficacy of inulin-type fructans. Previous studies suggest that prebiotic selectivity of inulin-type fructans towards bifidobacteria is unaffected by the food matrix. Due to differences in study design, definitive conclusions cannot be drawn from these findings with any degree of certainty. In this randomised trial, we aimed to determine the effects that different food matrices had on the prebiotic efficacy of inulin-type fructans following a standardised 10-day, 4-arm, parallel, randomised protocol with inulin either in pure form or incorporated into shortbread biscuits, milk chocolate or a rice drink. Similar increases in Bifidobacterium counts were documented across all four interventions using both fluorescence in situ hybridisation (pure inulin: +0.63; shortbread: +0.59; milk chocolate: +0.65 and rice drink: +0.71 (log10 cells/g wet faeces) and 16S rRNA sequencing quantitative microbiome profiling data (pure inulin: +1.21 × 109; shortbread: +1.47 × 109; milk chocolate: +8.59 × 108 and rice drink: +1.04 × 109 (cells/g wet faeces) (all P ≤ 0.05). From these results, we can confirm that irrespective of the food matrix, the selectivity of inulin-type fructans towards Bifidobacterium is unaffected, yet the compositional make-up of the food matrix may have implications regarding wider changes in the microbiota.
摘要：
最近，科学界对食品基质对菊粉型果聚糖的益生元功效的影响存在很多争论。先前的研究表明，菊粉型果聚糖对双歧杆菌的益生元选择性不受食物基质的影响。由于研究设计的差异，无法从这些发现中得出任何确定性的结论。在这项随机试验中,我们的目的是确定不同的食物基质对菊粉型果聚糖的益生元功效的影响，四臂，平行,随机方案，菊粉以纯形式或掺入脆饼饼干中，牛奶巧克力或米饭饮料。使用荧光原位杂交（纯菊粉：0.63；脆饼：0.59；牛奶巧克力：0.65和大米饮料：0.71（log10细胞/g湿粪便）和16SrRNA测序定量微生物组数据（纯菊粉：1.21×109；脆饼：1.47×109；牛奶巧克力：湿饮料：8.59×109。从这些结果来看,我们可以确认，无论食物基质如何，菊粉型果聚糖对双歧杆菌的选择性不受影响，然而，食物基质的组成组成可能会对微生物群的更广泛变化产生影响。
