Abstract：
OBJECTIVE: Vitamin B7(biotin) is not synthesized in our body and is retrieved from some food products like eggs, liver, pork and leafy vegetables and as well as microbes of gut. Deficiency of biotin majorly leads to loss of hair, rashes over skin, lethargy and seizures. It is noted that biotin is an anti-oxidant and negates free radical effects. Biotin is also involved in carbon dioxide metabolism and it might alter seizure threshold. Studies also suggest its effect on lipid metabolism as well. So, the primary objective of this study was to assess the efficacy of biotin in maximal electric shock (MES) induced generalized tonic-clonic seizures (GTCS) and pentylenetetrazole (PTZ) induced absence seizures. The secondary objective is to study the effect of combined treatment of biotin and sodium valproate on seizures as well as plasma lipid profile in rats.
METHODS: In our study 30 albino Wistar rats each were used in MES and PTZ model respectively. 30 rats were divided equally into following groups: I - distilled water (negative control) II - distilled water (positive control) III - sodium valproate (300 mg/kg) IV - biotin (10 mg/kg/day) V - biotin (10 mg/kg) + sodium valproate (150 mg/kg).
RESULTS: We observed that the tonic hind limb extension was significantly reduced in the treatment group in MES model. Nitric oxide levels were also seen raised in combination group in MES model and all the treated groups in PTZ model. Biotin treated group showed increased high-density lipoproteins and reduced low density lipoproteins and triglycerides.
CONCLUSIONS: Biotin had an additive effect to sodium valproate in both the models of epilepsy in rats. Further, it was also able to counteract hyperlipidemia cause by sodium valproate.
METHODS: In our study 30 albino Wistar rats each were used in MES and PTZ model respectively. 30 rats were divided equally into following groups: I - distilled water (negative control) II - distilled water (positive control) III - sodium valproate (300 mg/kg) IV - biotin (10 mg/kg/day) V - biotin (10 mg/kg) + sodium valproate (150 mg/kg).
RESULTS: We observed that the tonic hind limb extension was significantly reduced in the treatment group in MES model. Nitric oxide levels were also seen raised in combination group in MES model and all the treated groups in PTZ model. Biotin treated group showed increased high-density lipoproteins and reduced low density lipoproteins and triglycerides.
CONCLUSIONS: Biotin had an additive effect to sodium valproate in both the models of epilepsy in rats. Further, it was also able to counteract hyperlipidemia cause by sodium valproate.
摘要：
目的：维生素B7（生物素）不是在我们的身体中合成的，是从一些食物产品，如鸡蛋，肝脏,猪肉和多叶蔬菜以及肠道微生物。缺乏生物素主要导致脱发，皮肤上有皮疹，嗜睡和癫痫发作。注意到生物素是抗氧化剂并且消除自由基作用。生物素也参与二氧化碳代谢，它可能会改变癫痫发作阈值。研究还表明其对脂质代谢的影响。所以,本研究的主要目的是评估生物素在最大电击(MES)诱导的全身强直阵挛性癫痫发作(GTCS)和戊四氮(PTZ)诱导的失神发作中的疗效.第二个目的是研究生物素和丙戊酸钠联合治疗对大鼠癫痫发作以及血浆脂质分布的影响。
方法：在我们的研究中，30只白化病Wistar大鼠分别用于MES和PTZ模型。将30只大鼠平均分为以下组:I-蒸馏水(阴性对照)II-蒸馏水(阳性对照)III-丙戊酸钠(300mg/kg)IV-生物素(10mg/kg/天)V-生物素(10mg/kg)+丙戊酸钠(150mg/kg)。
结果：我们观察到，在MES模型中，治疗组的后肢伸展明显减少。在MES模型中的组合组和在PTZ模型中的所有处理组中也观察到一氧化氮水平升高。生物素处理组显示增加的高密度脂蛋白和减少的低密度脂蛋白和甘油三酯。
结论：在两种大鼠癫痫模型中，生物素对丙戊酸钠都有累加作用。Further,它还能够对抗丙戊酸钠引起的高脂血症。
方法：在我们的研究中，30只白化病Wistar大鼠分别用于MES和PTZ模型。将30只大鼠平均分为以下组:I-蒸馏水(阴性对照)II-蒸馏水(阳性对照)III-丙戊酸钠(300mg/kg)IV-生物素(10mg/kg/天)V-生物素(10mg/kg)+丙戊酸钠(150mg/kg)。
结果：我们观察到，在MES模型中，治疗组的后肢伸展明显减少。在MES模型中的组合组和在PTZ模型中的所有处理组中也观察到一氧化氮水平升高。生物素处理组显示增加的高密度脂蛋白和减少的低密度脂蛋白和甘油三酯。
结论：在两种大鼠癫痫模型中，生物素对丙戊酸钠都有累加作用。Further,它还能够对抗丙戊酸钠引起的高脂血症。
