Abstract：
Pit recharge systems (PRS) control odor by managing organic solids in swine manure. However, there needs to be more understanding of PRS\'s effect on the microbiome composition and its impact on odor formation. A study was conducted to understand how recharge intervals used in PRS impact manure microbiome and odor formation. Bioreactors dynamically loaded simulated recharge intervals of 14, 10, and 4 days by diluting swine manure with lagoon effluent at varying ratios. Treatment ratios tested included 10:0 (control), 7:3 (typical Korean PRS), 5:5 (enhanced PRS #1), and 2:8 (enhanced PRS #2). Manure microbial membership, chemical concentrations, and odorant concentrations were used to identify the interactions between microbiota, manure, and odor. The initial microbial community structure was controlled by dilution ratio and manure barn source material. Firmicutes and Proteobacteria were the dominant microbial phyla in manure and lagoon effluent, respectively, and significantly decreased or increased with dilution. Key microbial species were Clostridium saudiense in manure and Pseudomonas caeni in lagoon effluent. Percentages of these species declined by 8.9% or increased by 17.6%, respectively, with each unit dilution. Microbial community composition was controlled by both treatment (i.e., manure dilution ratio and barn source material) and environmental factors (i.e., solids and pH). Microbiome composition was correlated with manure odor formation profiles, but this effect was inseparable from environmental factors, which explained over 75% of the variance in odor profiles. Consequently, monitoring solids and pH in recharge waters will significantly impact odor control in PRS.
摘要：
坑补给系统（PRS）通过管理猪粪中的有机固体来控制气味。然而,需要进一步了解PRS对微生物组组成的影响及其对气味形成的影响。进行了一项研究，以了解PRS中使用的补给间隔如何影响粪肥微生物组和气味形成。生物反应器通过用不同比例的泻湖废水稀释猪粪，动态加载14、10和4天的模拟补给间隔。测试的治疗比例包括10：0（对照），7:3（典型的韩国PRS），5:5(增强型PRS#1),和2:8(增强型PRS#2)。粪肥微生物成员，化学浓度,和气味浓度被用来识别微生物群之间的相互作用，粪肥,和气味。最初的微生物群落结构由稀释比和粪肥仓来源材料控制。厚壁菌和变形杆菌是粪便和泻湖废水中的主要微生物门，分别,并随着稀释而显著降低或升高。关键的微生物种类是粪便中的梭状芽胞杆菌和泻湖废水中的假单胞菌。这些物种的百分比下降了8.9％或增加了17.6％，分别,每个单位稀释。微生物群落组成由两种处理控制(即，粪肥稀释率和谷仓源材料)和环境因素(即，固体和pH)。微生物组组成与粪便气味形成概况相关，但是这种影响与环境因素密不可分，这解释了超过75%的气味变化。因此,监测补给水中的固体和pH值将显著影响PRS的气味控制。
