Abstract：
Blood-contacting catheters play a pivotal role in contemporary medical treatments, particularly in the management of cardiovascular diseases. However, these catheters exhibit inappropriate wettability and lack antimicrobial characteristics, which often lead to catheter-related infections and thrombosis. Therefore, there is an urgent need for blood contact catheters with antimicrobial and anticoagulant properties. In this study, we employed tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) to create a stable hydrophilic coating under mild conditions. Heparin (Hep) and poly(lysine) (PL) were then modified on the TA-APTES coating surface using the layer-by-layer (LBL) technique to create a superhydrophilic TA/APTES/(LBL)4 coating on silicone rubber (SR) catheters. Leveraging the superhydrophilic nature of this coating, it can be effectively applied to blood-contacting catheters to impart antibacterial, antiprotein adsorption, and anticoagulant properties. Due to Hep\'s anticoagulant attributes, the activated partial thromboplastin time and thrombin time tests conducted on SR/TA-APTES/(LBL)4 catheters revealed remarkable extensions of 276 and 103%, respectively, when compared to uncoated commercial SR catheters. Furthermore, the synergistic interaction between PL and TA serves to enhance the resistance of SR/TA-APTES/(LBL)4 catheters against bacterial adherence, reducing it by up to 99.9% compared to uncoated commercial SR catheters. Remarkably, the SR/TA-APTES/(LBL)4 catheter exhibits good biocompatibility with human umbilical vein endothelial cells in culture, positioning it as a promising solution to address the current challenges associated with blood-contact catheters.
摘要：
血液接触导管在当代医学治疗中起着举足轻重的作用，特别是在心血管疾病的管理。然而,这些导管表现出不适当的润湿性和缺乏抗菌特性，这通常会导致导管相关的感染和血栓形成。因此,迫切需要具有抗微生物和抗凝血特性的血液接触导管。在这项研究中,我们使用单宁酸(TA)和3-氨基丙基三乙氧基硅烷(APTES)在温和条件下产生稳定的亲水涂层。然后使用逐层(LBL)技术在TA-APTES涂层表面上修饰肝素(Hep)和聚(赖氨酸)(PL),以在硅橡胶(SR)导管上产生超亲水性TA/APTES/(LBL)4涂层。利用这种涂层的超亲水性，它可以有效地应用于血液接触导管以赋予抗菌，抗蛋白吸附，和抗凝血性能。由于Hep\的抗凝特性,在SR/TA-APTES/（LBL）4导管上进行的活化部分凝血活酶时间和凝血酶时间测试显示，显着延长了276％和103％，分别,与未涂覆的商用SR导管相比。此外，PL和TA之间的协同相互作用有助于增强SR/TA-APTES/（LBL）4导管对细菌粘附的抵抗力，与未涂覆的商用SR导管相比，它减少了高达99.9%。值得注意的是,SR/TA-APTES/（LBL）4导管与培养的人脐静脉内皮细胞具有良好的生物相容性，将其定位为解决当前与血液接触导管相关的挑战的有前途的解决方案。
